Linkage Mapping Via Crosses

RFLP

RFLP

________: is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes.

SNP

________: are a type of polymorphism involving variation of a single base pair.

Allelic differences

________ between genes were used to map the relative locations of those genes along a chromosome by conducting test crosses.

Molecular markers

________ are polymorphic; within a population, they may vary from individual to individual.

diploid species

In a(n) ________, an individual has 2 copies of a given STS.

STS

________: Stretch of DNA that a PCR can easily detect.

Microsatellites

________: short repetitive sequences that are abundantly interspersed throughout a species genome and tend to vary in length among different individuals.

AFLP

________: Same as RFLP nut the DNA sequence being used is amplified via PCR instead of isolating the chromosomal DNA.

Distances

________ between linked molecular markers determine the outcome of crosses.

DNA fragment

The ________ in the higher band is more extended because it has more repeat sequences than does the lower band.

GH1

________: encodes acetolactate synthase.

DNA

When the ________ is collected from a haploid cell, an STS produces only a single band.

pair of PCR

If a(n) ________ primers copies a single site within a set of chromosomes, the amplified region is called a Sequence Tagged Site.

STS

When a(n) ________ contains a microsatellite, the PC products may be identical and result in a single band on a gel if the region is the same length in both copies.

Introduction

Definitions

Mapping

the experimental process of determining the relative locations of genes or other segments of DNA on individual chromosomes

Molecular marker

Segment of DNA that is found at a specific site along a chromosome and has properties that enable it to be uniquely recognized using molecular tools

RFLP

is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes

AFLP

Same as RFLP nut the DNA sequence being used is amplified via PCR instead of isolating the chromosomal DNA

SNP

are a type of polymorphism involving variation of a single base pair

STS

Stretch of DNA that a PCR can easily detect

RFLPs

Restriction fragment length polymorphism

Atc4

encodes actin

er

encodes erecta

GH1

encodes acetolactate synthase

Microsatellites

short repetitive sequences that are abundantly interspersed throughout a species genome and tend to vary in length among different individuals