CHEM402L: Atomic Absorption Spectroscopy

Atomic Absorption Spectroscopy

Technique for measuring metal ion concentrations.

Calcium Ion

Essential mineral measured at 422.7 nm wavelength.

Magnesium Ion

Essential mineral measured at 285.2 nm wavelength.

Parts Per Million (ppm)

Concentration unit for measuring dilute solutions.

EDTA Titration

Old method for determining calcium and magnesium.

Lanthanum (III) Ion

Counteracts phosphate ion interference in measurements.

Calibration Standards

Known concentrations used to create standard curves.

Intermediate Solutions

Diluted stock solutions for analysis preparation.

Blank Solution

Deionized water used as a reference sample.

Standard 1

5.00 ppm Ca2+ + 0.0625 ppm Mg2+ preparation.

Standard 2

10.00 ppm Ca2+ + 0.1250 ppm Mg2+ preparation.

Standard 3

12.50 ppm Ca2+ + 0.1875 ppm Mg2+ preparation.

Standard 4

15.00 ppm Ca2+ + 0.2500 ppm Mg2+ preparation.

Unknown Sample

Sample to determine unknown concentrations of ions.

Volumetric Flask

Glassware used for precise dilutions.

Absorbance Measurement

Determining light absorption by analytes in solution.

Calibration Plot

Graph of absorbance versus concentration for standards.

Linear Least-Squares Fit

Method for fitting linear data points.

Second-Order Polynomial Fit

Used for fitting calcium data in analysis.

Dilution Factor

Factor accounting for sample dilution in calculations.

Deionized Water

Purified water used in sample preparation.

Stock Solutions

Concentrated solutions used to prepare standards.

Experimental Measurements

Process of measuring absorbance with AA spectrophotometer.