1. Microscopy

Microscope

• A scientific instrument which can zoom in on an object, enabling the user to see it up close

Cell Theory

• Plants and animal cells tissues are composed of cells

• Cells are the basic unit of all life

• Cells only develop from existing cells

Function of the Lenses

• Objective - placed near the specimen, produces a magnified image

• Eyepiece - where the specimen is viewed

Resolution

• Resolution is the amount of detail seen in a specimen sample, so an increased resolution makes the object more distinct

Illumination

• Light is placed under the sample, opaque specimens can be viewed by a light above

Magnification Calculation

• Size of Image/Actual Size of Object

Magnification Conversions

• 1000 nanometres - 1 micrometre

• 1000 micrometres - 1 millimetre

• 1000 millimetres - 1 metre

Microscopy - Calibration

• Using an Eyepiece Graticule and a Stage Micrometer

• Align the micrometer scale with the eyepiece scale

• 100 divisions (E.G) - 1 mm (S.M)

1 Graticule Division Calculation

• Number of micrometres/number of graticule divisions

Measurement of the Sample Calculation

• Graticule Divisions x Magnification Factor