BLOCK 3 lecture 3

what do microbes need to get from outside the cell

membranes

facilitated diffusion

primary active transport

Primary always requires hydrolysis of ATP

secondary active transport

glucose transport in microbes

PEP is one of the 12 essential molecules of life

Some microbes can use this in a similar way to ATP as a phosphate donor

complex carbohydrates

types of enzyme secretion

Continuous channel spans both membranes of the bacteria

Goes into the periplasm and then out

Cellulases important

example - cellulosome

whole pathway secretion - OMVs

sensing and motility

Counterclockwise is run, clockwise is tumble

sensing and motility - tumble

Happens continuously when nothing is bound

sensing and motility - run

scavening metals

microbial predation

Prey is large and contains a lot of nutrients

Binds membrane of prey (type IV) and injects degrading enzymes into this bacteria which degrade the membrane of the prey to release the nutrients inside the cell so they are available to use for metabolism 

Still has to transport across the membrane