Staining

What color is Hematoxylin?

Purplish-blue

What type of structures does Hematoxylin bind to?

Basophilic

What color is Eosin?

pink to red

What type of structures does Eosin bind to?

acidophilic

What cell shape is Elongated, spindle-shaped cells tapering at both ends and elongated nuclei are visible, but cell boundaries are often hard to distinguish?

fusiform

What cell shape is Smooth muscle cells arranged in parallel bundles?

fusiform

What cell shape has multiple flattened surfaces, often appearing pentagonal or hexagonal.

polyhedral

What cell shape is Smooth muscle cells arranged in parallel bundles?

polyhedral

Have multiple flattened surfaces,

often appearing pentagonal or hexagonal.

What stain is commonly used in hematology for blood and bone marrow smears.

Wright-Giemsa stain

What dyes do wright-giemsa stain contain>

Eosin, methylene blue

What stain type highlights collagen and connective tissue? Also Distinguishes fibrosis/scar from muscle and parenchyma

Trichrome Stain

What stains are used in trichrome stain?

Masson, Mallory, Gomori

What is the typical color of collagen/dense connective tissue in trichrome stain?

blue, green

What is the typical color of muscle and cytoplasm in a trichrome stain?

red

What is the typical color of nuclei in a trichrome stain?

dark blue, black

Type of stain to identify

Periodic acid–Schiff reaction

What color do carbohydrates turn with PAS stain?

magenta

Digests glycogen to dertermin if PAS positve tissue has glycogen

diastase

Type of stain that visualizes reticular fibers (type III collagen) and certain nervous tissue structure such as neurofibrils, axons, glial processes

silver

tissues highlighted by silver stain

lymph nodes, spleen, liver, bone marrow septa, adipose tissue septa

What stain is used to distinguish elastic fibers from collagen fibers in connective tissue

Elastin

What color do elastic fibers turn under elastin?

brown, dark purple

What color do collagen fibers turn under elastin?

none

List 3 lipid stains

oil red O, sudan black, osmium tetroxide

What color does osmium tetroxide stain lipids in myelin sheath?

black

What color does sudan black stain lipids?

black

What color does oil red Os stain lipids?

red