Aerobic Gram Positive Bacilli

What 2 aerobic GPR genera are catalase + and usually motile?

1. Bacillus

2. Listeria

What GPR forms spores?

Bacillus species

What is the colony morphology of Bacillus species?

large, flat, feathered edge, usually beta hemolytic

What genus is this?

Bacillus (GPR with spores)

What aerobic GPR genus is a frequent lab contaminant in cultures?

Bacillus

What species of Bacillus is found in meat, poultry and fried rice?

B. cereus

What species of Bacillus causes food poisoning?

B. cereus

What species of Bacillus forms long chains and has a “bamboo like” appearance?

B. anthracis

What organism is shown in the Gram stain?

B. anthracis (GPR with spores and long chains)

Which Bacillus species is gamma hemolytic on SBA?

B. anthracis

B. anthracis causes what disease in cattle?

Anthrax

How does an Anthrax infection appear in humans?

blackened dead tissue (Anthrax means coal”)

What is the colony morphology of Listeria monocytogenes?

small, translucent gray, small zone of beta hemolysis

What aerobic GPR genus is a very small “coccobacillus” and may resemble GPC when Gram stained?

Listeria

What aerobic GPR genus is known to have “tumbling” motility?

Listeria

What aerobic GPR genus is fount in cabbage, raw fruits, deli meats, and hot dogs?

Listeria

What aerobic GPR genus is a psychrophile and grows will at 4C?

Listeria

Who is at the highest risk for infections caused by L. monocytogenes?

pregnant women

What aerobic GPR genus causes meningitis in immunocompromised patients?

Listeria

What aerobic GPR genus is BEA + and CAMP +?

Listeria

What aerobic GPR genus is catalase + and nonmotile?

Corynebacterium

What aerobic GPR genus is described as “clubs” or “diphtheroid” and form “L,” V,” and “Y” shapes when Gram stained?

Corynebacterium

What is the colony morphology of Corynebacterium species?

small, white/gray, gamma hemolytic (resembles CoNS)

What aerobic GPR genus is commensal flora of our skin and mucous membranes?

Corynebacterium

What species of Corynebacterium has a beaded appearance when stained with what stain?

C. diphtheriae, methylene blue stain

What species of Corynebacterium is NOT considered part of our normal flora on our skin and mucous membranes?

C. diphtheriae

Modified Tinsdale agar and Tellurite agar are (selective/nonselective) mediums used to grow what organism?

selective, C. diphtheriae

C. diphtheriae will have what appearance on the Tinsdale and Tellurite agars?

black colonies

Modified Loeffler’s medium is a (selective/nonselective) medium used to grow what organism?

nonselective, C. diphtheriae

What test is used to confirm if the cultured C. diphtheriae is a toxin producing strain?

Elek

What species of Corynebacterium colonizes our skin and causes skin infections?

C. jeikeium

What 2 aerobic GPR genera are catalase - and nonmotile?

1. Lactobacillus

2. Arcanobacterium

Which catalase - and nonmotile aerobic GPR is alpha hemolytic?

Lactobacillus species

What aerobic GPR genus is part of the commensal flora of the female genital and GI tract and oral cavity?

Lactobacillus

What aerobic GPR genus exhibits long, thin chains with the occasional spiral when Gram stained?

Lactobacillus

What is the colony morphology of Lactobacillus species?

tiny, alpha hemolytic

Which catalase - and nonmotile aerobic GPR is beta hemolytic?

Arcanobacterium species

What aerobic GPR genus causes pharyngitis and wound infections?

Arcanobacterium

What aerobic GPR genus is catalase - and H2S +?

Erysiopelothix

What aerobic GPR genus is known as the swine pathogen and is an occupational hazard to those that work with animals?

Erysipelothrix

What aerobic GPR genus causes red skin lesions?

Erysipelothrix (lesions are called Erysipeloids)

What 2 organisms are in the Actinomycetes family?

1. Nocardia

2. Rhodococcus

What is the colony morphology of Nocardia?

chalky, dry, crumbly

What aerobic GPR genus is a branching GPR and is partially acid-fast?

Nocardia

What aerobic GPR genus causes cutaneous infections known as mycetoma?

Nocardia

What is the colony morphology of Rhodococcus?

mucoid, pink

What is the morphology of Kurthia species?

white, smooth