Qualitative Tests (Cardiac glycosides)

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Cardiac Glycosides

Last updated 4:39 PM on 9/4/26
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5 Terms

1
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Keller Killiani Test

  • Presence of Deoxy Sugar

  • Result: BLUE or VIOLET coloration after standing


2
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Liebermman Burchard Test

  • Unsaturated Sterol

  • Conc. H2SO4 + Acetic anhydride

  • Result: GREEN, BLUE ( PALE YELLOW if saturated sterol)


3
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Salkowski’s test

  • Sterol (CPPP)

  • Conc. H2SO4

  • Result: RED or VIOLET


4
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Kedde’s Test

  • Unsaturated Lactone

  • 3,5-Dinitrobenzoic acid

  • Std: 0.025% digitoxin in MeOH

  • Result: BLUE-VIOLET


5
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  1. Keller Killani’s Test

  2. Salkowski’s Test

  3. Kedde’s Test


Considered a cardiac glycoside if they test positive for the following tests