molds

Filamentous fungi

molds

Molds basic structural unit is

hypha

Molds Reproduces by

conidia

Yeasts vs. Molds

yeast are unicellular and white in color and reproduces by budding and forms smooth colonies while molds are multicellular and multicolored and reproduces by spores and fuzzy colonies

Classic pigment & morphology, but not necessarily

the best primary growth or sporulation.

sda

⮚ Enhances reproductive structures production

potata dextrose agar

⮚ Enhances growth of dimorphic fungi

BHIA)

Partially differential and selective for dermatophytes.

dermatophyte test medium

✔1st week: Every day

rapid growers

✔2nd week: Every 2-3 days

✔3rd – 4th week: Once a week

slow growers

Microscopic methods for examination of Fungi:

1. Tease mount technique

2. Scotch tape method/Cellophane tape method

3. Riddell Culture Method / Microculture method

Widely used method for staining and observing fungi

tease mount technique

tease mount technique disadvantage

morphology not intact

Compared to Tease mount technique, morphology appears intact.

Cellophane Tape Mount

Cellophane Tape Mount disadvantage

Only superficial structures of fungi tend to stick to the tape.

This is the best method for the microscopic examination of

mold cultures.

slitde culture (riddell) technique

4 shapes of conidia

globose ovate elliptical

Septations

ameroconidia

1 celled

didymoconidia

2 celled

phragmoconidia

multicelled (transverse)

dictyoconidia

multicelled (longitudinal)

Arrangement of conidia

acropetal

youngest at the tip

basipetal

youngest at the base