UCF General Microbiology: Lab Practical

is in a broth

This technique is used in a culture that

air drying

This is an example of

simple stain

There are both gram negative and gram positive cocci on the picture. This stain is a

crystal violet, or any cationic dye

Fill in the blank:

negative stain

This procedure is for the

nigrosin

The second picture's dye is most likely

amount of peptidoglycan

What determines the gram stain result is the

gram stain

These agents are used in the

gram negative

These are

gram positive

These are

staphylcoccus

This is

streptococcus

This is

bacilli

This is gram negative

streptobacilli

This is gram negative

vibrio

This is an example of

spirilium

This is an example of

coccobacillus

Morphology:

club-shaped

Morphology:

acid-fast-positive organisms

These are examples of

free spores and endospores

The green are

vegetative cells

The pink are

spore-forming organisms

These are

anaerobic

Clostridium is

aerobic

Bacillus is

acid-fast and spore staining

This procedure is for

acid-fast stain

This procedure is for the

carbol fuschin

Primary dye for this procedure:

spore stain

This procedure is for the

malachite green

Primary dye for this procedure:

pour-plate method

This isolation method is the

3-zone streak plate

This is a

primary streak

These both come from the same broth. The first plate is the

secondary streak, or subculture

These both come from the same broth. The second plate is the

surface colony

Looking from the top, the black arrow points to a(n)

embedded colony

Looking from the top, the purple arrow points to a(n)

bottom colony

Looking from the top, the pink arrow points to a(n)

one cell

The assumption is that each isolated colony in zone 3B comes from

using a fourth zone

Error in streaking:

inadequate streaking

Error in streaking:

slashing the agar

Error in streaking:

making zones too large

Error in streaking:

crossing over into other zones during final streak

Error in streaking:

insufficient streaking in final zone

Error in streaking:

loop angle too flat on agar

Error in streaking:

selective for gram positive

not differential

phenyl ethyl alcohol inhibits gram negatives

PEA

Selective:

Differential:

Dyes/Reagents:

selects for gram negative

defferentiates lactose fermenters

eosin Y, methylene blue inhibit gram positives

EMB

Selective:

Differential:

Dyes/Reagents:

metallic green sheen

On EMB, E. coli produces a

pale pink to lavender center

On EMB, E. aerogenes produces a

not a lactose fermenter

Based on the picture, P. aeruginosa is

selects for gram negative

differentiates lactose fermenters

neutral red is absorbed by lactose fermenters

desoxycholate inhibits growth of gram positives

DES

Selective:

Differential:

Dyes/Reagents:

selects for gram negative

differentiates lactose fermenters

neutral red absorbed by lactose fermenters

bile salts, crystal violet inhibit gram positives

MAC

Selective:

Differential:

Dyes/Reagents:

not selective

differentiates based on RBC hemolysis

5% sheep RBC used as hemolysis indicator

Blood Agar

Selective:

Differential:

Dyes/Reagents:

beta hemolysis

Organisms with the clear ring exhibit

alpha hemolysis

Organisms with the cloudy ring exhibit

gamma hemolysis

Organisms with no ring exhibit

motility test

This is an example of a

tetrazolium chloride

The dye in this procedure is

not selective

differentiates based on lipase production

spirit blue dye around the growth indicates lipase production

Lipase Plate

Selective:

Differential:

Dyes/Reagents:

not selective

differentiates caseinase producers

casein in the milk is broken down, made clear in place of caseinase

Milk Agar

Selective:

Differential:

Dyes/Reagents:

not selective

differentiates amylase producers

iodine added to edge of growth. if it has a clearing around organism, positive for amylase

Starch Agar

Selective:

Differential:

Dyes/Reagents:

litmus milk

These tubes are filled with

acid production in litmus milk

These are examples of a(n)

alkaline production in litmus milk

This indicates a(n)

reduced condition in litmus milk

The white indicates a(n)

oxidized condition in litmus milk

The color at the top indicates a(n)

proteolysis from production of caseinase

What is happening to make the medium clear:

lactose fermentation that produces acids strong enough to denature the proteins

What causes the litmus milk to curd:

PR sugar broth

These are the results for the

phenol red

Indicator in these tubes:

yellow, fermentation of sugar to acid (bubble present if gas formed)

Positive result color, interpretation:

cerise (pink), peptone degredation increases pH by releasing ammonia

Negative result color, interpretation:

KIA/RDS media

These tubes are results from

lactose and glucose fermentation

Interpretation of the 2nd tube:

lactose fermentation by 2,3 butanediol fermenter, reversion from unstable acid products

Interpretation of the 3rd tube:

glucose fermentation and peptone degradation

Interpretation of the 4th tube:

only KIA media

glucose fermentation only and sulfur reduction

Interpretation of the 5th tube:

lactose and glucose fermentation

gas produced

Interpretation of the 6th tube:

MR test

These are the results for

methyl red

Reagent for this test:

indicates mixed acid fermenters

Purpose of this test:

2,3 butanediol fermenters

This tests for:

VP test

These are the results for

AMC produced by 2,3 butanediol fermenters

The reagents in this broth react with

VP I (alpha napthol) and VP II (KOH)

Reagents with this test:

nitrate test

This is the

nitrate reductase

This tests for

Nitrate I (sulfanilic acid) and Nitrate II (dimethyl-alpha-napthylamine)

Zinc added as a catalyst

Reagents for this test, what it :

B. cereus, P. aeruginosa

Based on the picture, which organism(s) has nitrate reductase:

L. lactis

Based on the picture, which organism(s) does not have nitrate reductase:

urea broth

This is the

P. vulgaris

Organism with positive result:

gelatinase

This test detects:

must put in ice bath

Special instructions with this test:

Simmons Citrate slant

This is the

growth

Primary indicator in this test:

use of citrate as sole carbon source

This medium detects

bromothymol blue

Indicator in this medium:

phenylalanine slant

This is the

phenylpyruvic acid (PPA), which is a byproduct of the breakdown of phenylalanase

The reagent added to this test indicates presence of

P. vulgaris

Organism positive for phenylananine deaminase: