MicroLab Practical

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"Oh boy"

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10 Terms

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Staphylococcus epidermis

Gram-positive

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Escherichia coli

Gram-negative

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Gram-positive

Thicker layer of peptidoglycan

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Gram-negative

Thinner layer of peptidoglycan

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First Step

Primary Stain: Crystal Violet

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Second Step

Mordant: Gram’s Iodine

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Third Step

Decolorizing Agent: Acetone-alcohol

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Fourth Step

Counterstain: Safranin

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Gram-positive

Purple (Crystal Violet)

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Gram-negative

Pink or Red (Safranin)