Principles of Molecular and Immunologic Studies - Alternative Amplification Techniques

These flashcards cover key vocabulary and concepts related to alternative amplification techniques in molecular and immunologic studies.

Ligase Chain Reaction (LCR)

A method that uses ligase and polymerase for probe amplification, allowing for the detection of specific mutations in gene sequences.

Strand Displacement Amplification (SDA)

An isothermal amplification method that allows simultaneous amplification and detection of nucleic acids.

Transcription-Mediated Amplification (TMA)

A technique where RNA is the target and product, involving reverse transcription to create cDNA.

Nucleic Acid Sequence-Based Amplification (NASBA)

A method of amplification that involves synthesizing RNA from a DNA template through reverse transcription and transcription processes.

Branched DNA (bDNA)

A signal amplification method that uses probes to detect specific nucleic acid sequences without replicating them enzymatically.

Hybrid Capture (HC)

An amplification technique that uses antibodies to capture RNA:DNA duplex molecules for detection.

Cleavase Based - Invader Assay

A method that allows simultaneous detection of different alleles using allele-specific probes and a cleavage enzyme.

Target amplification

The process of exponentially increasing the quantity of a specific nucleic acid sequence for analysis.

Signal amplification

A technique that increases the sensitivity of detection by amplifying the signal rather than the product.

Polymerase

An enzyme that synthesizes nucleic acids from a DNA or RNA template by adding nucleotides.

Restriction Enzyme

An enzyme that cuts DNA at specific recognition sequences, used in molecular biology for cloning and gene manipulation.