MI - Unit 1.1 Quiz

Preventative Interventions

Prevent problems in future

Diet

Vitamins

Therapeutic Interventions

Treat existing problems

Outbreak

Sudden rise in the incidence of a disease

Pathogen

A specific agent of a disease

Bioinformatics

The collection, classification, storage, and analysis of biochemical and biological information using computers especially as applied in molecular genetics and genomics

Genome

The complement of an organisms genes; an organisms genetic material

What color is G

BLACK

What color is A

GREEN

What color is T

RED

What color is C

BLUE

PCR

Using some fluorescent tagged nucleotides (produces DNA copies of differing lengths)

What does ELISA stand for

Enzyme

Linked

Immune

Sorbet

Assay

Step 1 ELISA

Experimental sample is added to the wells

Step 2 ELISA

Detergent washes out unbound protein + blocks remaining surface of wells

Step 3 ELISA

Primary antibody binds to a specific antigen

Step 4 ELISA

Unbound primary antibody is washed away

Step 5 ELISA

Secondary antibody, that has an enzyme linked colormetric tag attached, is added to the wells and binds to the primary antibody

Step 6 ELISA

Excess secondary antibody is washed away

Step 7

substrate is added to the wells

Step 8 ELISA

The substrate binds to the enzyme bound to the secondary antibody, causing a color change

Step 9 ELISA

A color change indicates that the antigen being tested for is present in the sample