Microscope

A parfocal microscope is one that keeps the specimen in focus (or very close to it) when a higher-power objective lens is rotated into position, after focusing with a lower-power objective.

true

The greatest magnification on the compound light microscope can be achieved by using the high power objective lens.

true

When viewing a specimen on a compound light microscope, the image is right-side-up but reversed.

true

While looking into the ocular lens to observe a specimen on a prepared slide at scanning power, you see only a part of the field of view. What can you do to solve this problem?

Use the mechanical stage controls to move the prepared slide so that it is centered in the partial field of view.

Which of the following parts of the microscope concentrates the light onto the microscopic slide?

Condenser

The field of view when using the high-power objective is smaller than when using the low-power objective.

true

A compound microscope is one that has two ocular lenses.

false

Which part of the microscope is responsible for making small changes in focus when using the high-power objective lens?

fine adjustment knob

What is the working distance on a microscope?

The distance between the objective lens and the slide

What is the proper way to carry a microscope?

Use one hand on the arm of the microscope, and the other hand supporting the base.

To put a specimen on the slide in the center of the field of view, what microscope part should be used?

mechanical stage controls

The magnification of a specimen viewed with a 10x eyepiece and a 40x high-power objective lens is _________

400x

If a microscopic lens is not clean, after cleaning it with lens paper or a cotton swab and lens cleaner, it should be cleaned with hydrochloric acid.

false

All of the following practices should be followed when making a wet mount, except

if using the oil immersion lens, open the iris diaphragm to illuminate the specimen.

What microscope part can be used to control light intensity and image contrast?

iris diaphragm lever

What is the typical magnification provided by the eyepiece (ocular) lens system?

10x

Why is it important to use only the fine adjustment knob when using the high-power or oil immersion lenses?

Using the coarse adjustment knob might crack the coverslip or slide, due to the close working distance.

All of the following are true of a stereomicroscope, except

• the image of the specimen is reversed and inverted.

Complete each statement by providing the correct microscope part that is being described.

1. The_____alters the focus of the eyepiece to compensate for vision differences between the two eyes.

2. The_______ can be used to control image contrast; for example, if the microscope slide specimen appears too dark, then this can allow more light to enter the condenser.

3. Under high-power objective, the only knob that should be used to focus is the _____

4. The knob used to control the brightness of light is the________

5. When using a binocular microscope, both eyes should be open when looking into the_______

diopter adjustment ring, iris diaphragm, fine focus knob, rheostat, eyepieces

calculations

1. When the scanning (4X) objective is used the total magnification will be

2. When the low power (10X) objective is used the total magnification will

3. When the high power (40X) objective is used the total magnification will

4. When the oil immersion (100X) objective is used the total magnification

40, 100, 400, 1000

The _________causes the stage to move upward or downward.

adjustment knob

know this

microscope

The _________blank are knobs that cause the microscope slide on the stage to move forward and backward and side-to-side.

mechanical stage controls

scanning objective, low power objective, high power objective

Select the correct word(s) using the drop-down list that correctly complete each statement. (Not all labels will be used.)

1. When looking through to make observations, the (Click to select) condenser scanning ocular lens arm high-power eyepiece coarse adjustment knob fine adjustment knob stage clips low-power magnifies the image 10x.

2. If the specimen is appearing too dark, the light entering the (Click to select) coarse adjustment knob high-power scanning stage clips low-power eyepiece fine adjustment knob condenser ocular lens arm can be adjusted by opening the diaphragm using the iris diaphragm lever.

3. The (Click to select) low-power arm fine adjustment knob stage clips ocular lens scanning coarse adjustment knob high-power eyepiece condenser is used to get scanning and low-power objectives in focus.

4. The (Click to select) condenser coarse adjustment knob scanning high-power arm fine adjustment knob eyepiece stage clips ocular lens low-power objective provides the greatest magnification, while the scanning objective provides the least magnification.

5. The eyepiece is also called the (Click to select) coarse adjustment knob condenser low-power arm eyepiece high-power scanning stage clips fine adjustment knob ocular lens , and it is used to calculate total magnification of an objective lens.

6. Scanning and then (Click to select) scanning high-power low-power arm stage clips coarse adjustment knob condenser ocular lens fine adjustment knob eyepiece objectives are used to get a specimen in focus before using high-power.

7. If the slide is moving as you try to center it in the field of view, check that you have secured the slide to the stage using the (Click to select) fine adjustment knob coarse adjustment knob low-power stage clips high-power arm condenser scanning eyepiece ocular lens .

8. The flat surface that supports the microscope is the base, and it, along with the (Click to select) arm low-power ocular lens eyepiece scanning fine adjustment knob high-power stage clips coarse adjustment knob condenser requires one hand on each part to help carry the microscope.

9. When observing the spinal cord in a prepared slide, the (Click to select) arm fine adjustment knob condenser eyepiece ocular lens scanning coarse adjustment knob low-power stage clips high-power magnification would be used to see the largest amount of the specimen in the field of view.

eyepiece, condenser, coarse adjustment knob, high power, ocular lens, low power, stage clips, arm, scanning