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What sample type is collected in a red top?
Serum
What sample type is collected in a green top?
Plasma
What chemical assays must be put on ice?
Ammonia
Blood gas
Lactic acid
What is the additive and anticoagulant in grey top?
Additive: Sodium fluoride
Anticoagulant: Potassium oxalate
What test is done using a grey tube?
Glucose tolerance test (GTT)
Fasting blood sugar
Blood alcohol
Lactic acid
Why must EDTA tubes not be used for chemistry tests? What measurements are falsely increased and decreased?
EDTA contains sodium and potassium which will lead to falsely elevated results, and it chelates calcium ions which will falsely decrease calcium results
What measurements are affected by running a hemolyzed sample? (Hint: KLAMP)
Elevated Potassium, LD, ALD, ACP, Magnesium, Phosphate, and hemoglobin
What causes hemolysis during collection?
Small needle diameter, tourniquet bound for too long, rough pull on syringe, prolonged testing, improper temperature during storage and transport