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Step 1:
Building blocks (NAG, NAM, amino acids) are synthesized
Step 2:
NAG-NAM-peptide created in cytoplasm
Step 3:
NAG-NAM-peptide precursor exported to periplasm
Step 4:
Transglycosylase PBP enzyme connects NAG-NAM to existing sugar chain
Step 5:
Transpeptidase PBP enzyme connects peptides into peptide crossbridge