Blood Collection

Tests that require peripheral smear

• Bleeding Time

• Clotting time

Type of EDTA not suitable for Hematological exam

Tripotassium EDTA

Dipotassium

Disodium

calcium

Which of the following anticoagulants is considered as the best anticoagulant for blood transfusion, non-toxic, and is easily eliminated from the body

Lithium Heparin.

A. 3.8% Sodium Citrate : light blue top

B. 3.2% Sodium Citrate : black tube top

Neither are associated

Paul Heller Fluid

aka Ammonium and potassium oxalate

Serum tube that contain w/ or w/o clot activator or gel

1. Red top

2. Gold top

3. Mottled or tiger top

Skin puncture for patients—

w/ severe burns

obese

a patient with DIC

A. Thrombocytosis : occur during exercise

B. Thrombocytopenia : occur during cellular clumping

Correct order of draw w/ the ff. requests: FBS, CBC, and blood gas analysis

green - pink/lavender - gray

If the phlebotomist has no other alternative, than to extract from an IV site, he should ask the nurse to stop the infusion for a period of:

2 mins before drawing specimen

A. Tan evacuated tube (plastic): K2EDTA for Hematologic axam

B. Pink evacuated tube : K2EDTA for blood banking

Serum is hemolyzed in the presence of:

• Increased hemoglobin in a non-fasting sample

• Increased lipids in a non-fasting sample

• Increase hemoglobin due to the ruptured of R B C

• Increased carbon dioxide from cell metabolism

A. Exercise : Increase Blood Clotting factors

B. Stress : Increase blood clotting factors

1. Gray Troop used for blood lactate test

2. Lavender tube used for RBC Indices

3. gray tube used for blood alcohol test

4. Red tube used for many tests, including serum enzymes tests.

2, 3, and, 4

A. Increase Cortisol Hormone : during afternoon

B. Increases Cortisol hormone : Smokers

A green evacuated troop cannot be used to collect specimen for

1. Morphological study of Platelet and WBC

2. Blood pH measurement

3. Electrolytes measurement

4. Blood Ammonia measurement

A. Disadvantage of Heparin Anticoagulant : causing cellular clumping

B. Disadvantage of K2EDTA : causing cellular clumping

Sodium Citrate to Blood ratio

1:9

Tube top for Blood banking/crossmatching

Pink Top

A. Tube with thixotropic gel: moa prevent contamination of the serum or plasma with cellular components

B. Glass tube with silica particles: moa promotes blood clotting.

Versene and sequestrene are also known as

EDTA

A. Obese patient : median vein (recommended site)

B. pediatric patient : plantar side of heel

Only B

Which of the following is not true about 3.2% sodium citrate containing tubes?

Blood to anticoagulant ratio is 4:1

What additives may be used in hematology?

Polymer gel

Thrombin

Trisodium Citrate

A. EDTA Anti quarrel: for ESR Westergren Method

B. Buffered sodium citrate anticoagulant: for ESR Westergren Method.

A. Tourniquet : will distend a vein

B. Tourniquet : restricts blood flow

Which of the following evacuated troops used for blood serum collection:

1. gold top tube

2. orange top tube

3. Pearl top tube

4. yellow non-sterile tube

Which of the following will be observed if a purple top collection tube is underfilled?

Falsely decrease HCT

A. Anti glycolytic tube colonial sodium fluoride with potassium oxalate.

B. Light or dark green tube : ammonium hepper in a polymer gel.

Only A

A. SST : mixing 5 times

B. PST gel separator tube w/ heparin : 8 times

Both

When blood is collected into a tube containing thrombin and centrifuge after a collection, the top layer is.

serum

A. Tan top tube : used for lab determination

B. White top tube : for molecular testing.

Both

A. Royal blue evacuated tube : with sodium heparin

B. Green evacuated tube: w/ lithium heparin or sodium heparin.

When collecting a series of tubes during phlebotomy, which evacuated tubes should be filled last:

light green top, red top, lavender top, light blue

Lavender top.

A. Hemo concentration: causing shortened coagulation time test

B. Hemolysis: causing prolonged in APTT.

What anticoagulant not suitable for coagulation and platelet function tests?

Heparin

A. gray top tube: inhibit glycolysis.

B. Great top tube : inhibit blood clothing.

Which of the following evacuated troops with dipotassium salt?

1. White top tube w/ polymer gel.

2. Tan top tube.

3. Pink top tube.

4. Royal blue top tube.

What anticoagulant causes the least interference in plasma and whole blood chemistry assay

Lithium Heparin

A. White evacuated tube: binds calcium

B. Blue evacuated tube : binds in calcium

correct order of draw using capillary collection tube

Blood culture

EDTA

other Anticoag

Clot activators

Failure to become familiar with needle equipment can result in.

Accidental needle stick.

A. Nonstarial yellow or red plastic tube : w/ clot activator.

B. Gold top tube : gel separator

A. Pink top tube : MOA chelates calcium

B. Green top tube : MOA inhibits thromboplastin

In a multiple drug to obtain samples for CBC and electrolytes, which should be drawn first?

A. lavender top

B. green top

A. Green top tube: used for osmotic fragility test.

B. Green top tube: used for ammonia measurement.

Both

An evacuated tube containing excess EDTA can not be used to collect specimen for

1. Platelet count

2. ESR

3. RBC indices /MCHC

4. Retic count

What is the anticoagulant of choice for platelet function test or hemostasis test?

Light Blue

Heloysis

A. Orange top: thrombin

B. Gold top : gel separator