Studied by 2 peopleDeoxyribonucleic acid
A complex molecule found in nearly all cells in all animals and plants.
Polymerase Chain Reaction
A technique that is used primarily to increase the amount of DNA by amplification.
Homozygous
It is if a person inherits the same form of a gene from the mother and the father.
Mitochondria
These are small structures located within practically all animal and plant cells.
Phenotype
Observed characteristic expressed by the gene.
Heterozygous
If the person receives different forms of the same gene (A and B)
Genotype
The alleles that make up that gene.
DNA
When _____ degrades, long strands may become fragmented.
Probe Hybridization
Used to visualize the VNTRs.
Restriction fragment length polymorphism
The first DNA typing method to be widely adopted by forensic biologists in the analysis of crime.
Polymer
A molecule made up of repeating simpler units, called monomers.
extraneous source
If care is not used in DNA extractions and typing, then biologic material from a(n) ______, such as the evidence technician, scientist, or laboratory technician, can be introduced accidentally.
Denaturation
The DNA is added to the PCR tube that contains the reaction mixture and is then heated to 94°C.
Genes
Sections of chromosomes that function in inheritance and the development of an embryo after conception.
Genetic Concordance
The relationship between two samples of DNA.
Allele
Each form of a particular gene at a particular locus in the genome.
Reverse dot blot
Process involves identifying the particular alleles present by reacting them with color- forming reagents on specially treated nylon strips.
RFLP
In ______, DNA is extracted from biologic material and then restriction enzymes are used to isolate small fragments of DNA called minisatellites or variable number tandem repeats (VNTRs) using restriction enzymes.
DQ alpha
______ and a number of other genes collectively called polymarker are typed using a method called reverse dot blot.
Cells
______ contain many mitochondria and each one has up to 10 copies of mtDNA.
forensic analysis
The goal of all ______ is to associate a piece of evidence with as few people or objects as possible, and ideally for there to be only one possible source.
STR markers
_____ exhibit high variability in a population, thus giving rise to high degrees of association of evidence with a suspect.
STRs
_____ have certain advantages over VNTRs that make them attractive for forensic comparison purposes.
Short Tandem Repeats
Combines some of the attributes of both PCR and RFLP.
Nuclear DNA
It has a geometric shape called a double helix.
MtDNA
It is circular in shape, containing 26, 569 base pairs and codes for 37 genes.
Helix
A spiral-shaped geometric figure.
CODIS system
The ________ consists of types of three databases categorized by the type of information they contain.
DNA
It is a remarkably stable substance, but it can degrade from a number of causes.
Polymerase chain reaction
Had been used since the 1970s for making copies (amplifying) of DNA using polymerase enzymes.
Short Tandem Repeats
These have the same basic arrangement as VNTRs in that they contain repeating units of base pair sequences in tandem.
DNA
It is located in two regions in a cell, the nucleus and mitochondria.
1100
MtDNA contains a noncoding region of _____ base pairs that, in turn, contains two hypervariable regions.
Adenine, Guanine, Cytosine, and Thymine
Four bases or nucleotides
Homozygous
If a person inherits the same form of a gene from the mother and the father
Heterozygous
If the person receives different forms of the same gene (A and B)
Allele
Each form of a particular gene at a particular locus in the genome
Phenotype
Observed characteristic expressed by the gene
Genotype
The alleles that make up that gene
Tandem Repeats
The repeats are right next to each other, without any intervening base pairs
Restriction fragment length polymorphism (RFLP)
The first DNA typing method to be widely adopted by forensic biologists in the analysis of crime
Polymerase Chain Reaction (PCR)
A technique that is used primarily to increase the amount of DNA by amplification
Denaturation
The DNA is added to the PCR tube that contains the reaction mixture and is then heated to 94°C
Annealing
The next step in the PCR process is to attach a short strand of synthetic DNA to each of the separated strands
Human Leukocyte Antigen (HLA) DQ Alpha
First DNA region that was widely subjected to amplification and typing for forensic purposes by PCR
DQ Alpha
This gene exhibits sequence polymorphisms
Combined DNA Index System (CODIS)
The generic term used to describe the FBIs program of support for criminal justice DNA databases as well as the software used to run these databases
Note
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