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What is the main purpose of impregnation in histology?
To replace the clearing agent with a medium that provides a firm consistency.
Which of the following is NOT a type of tissue impregnation and embedding medium?
Acetone.
What is the most commonly used infiltrating and embedding medium in histology?
Paraffin wax.
Which of the following is an advantage of paraffin wax impregnation?
Allows rapid preparation of sections within 24 hours.
What is the recommended immersion time for tissues in paraffin wax to prevent shrinkage and hardening?
Not more than 4 hours.
Which of the following is NOT a method of paraffin wax impregnation?
Electrochemical processing.
What is the function of the vacuum embedding method?
To speed up infiltration by removing air bubbles and clearing agents.
In manual processing, how many changes of paraffin wax are required for proper impregnation?
Which automatic processing machine is commonly used in tissue impregnation?
Autotechnicon.
What is the routine melting point of paraffin wax used in histology?
56°C.
Which substitute for paraffin wax is commonly used for embedding neurological tissues and hard tissues like bones and teeth?
Celloidin.
What is the primary disadvantage of celloidin embedding?
Takes a long time for impregnation (several days to weeks).
Why is gelatin impregnation rarely used?
It is water-soluble and difficult to section.
Which embedding method is best for whole eye sections?
Dry celloidin method.
What is the purpose of embedding in histology?
To provide a support medium for sectioning.
What is the primary precaution to take when using vacuum embedding?
Avoid overheating to prevent tissue shrinkage.
Which embedding mold is adjustable and allows different tissue sizes?
Leuckhart’s Embedding Mold.
What is the primary advantage of the Tissue-Tek system?
Provides rapid embedding with easy orientation.
Which substitute for paraffin wax is commonly used for embedding undecalcified bones?
Methyl Methacrylate (MMA).
Which of the following describes the double-embedding method?
Uses a combination of celloidin and paraffin embedding.
What happens if tissue is inadequately infiltrated during impregnation?
It retains the clearing agent and remains soft.
Which of the following is the best embedding medium for routine histological procedures?
Paraffin wax.
Which clearing agent is easiest to remove during paraffin impregnation?
Benzene.
Which of the following is a disadvantage of paraffin wax embedding?
Cannot be used for enzyme histochemistry.
What is the recommended embedding method for fatty tissues?
Water-soluble wax.
What is the ideal melting point of paraffin wax used in histology when the lab temperature is 20-24°C?
54-58°C.
How many wax changes are required in manual processing for complete impregnation?
What is the function of the vacuum embedding technique?
To speed up impregnation and remove air bubbles.
Which embedding medium is recommended for processing whole eye sections?
Celloidin.
What is the purpose of using a cold plate during embedding?
To solidify the embedding medium rapidly.
Which processing method uses an automatic tissue processor?
Autotechnicon processing.
Which of the following is an advantage of the Tissue-Tek system?
Faster embedding and easier tissue orientation.
Which embedding mold consists of two L-shaped strips of heavy brass?
Leuckhart’s embedding mold.
Which embedding method is best for hard tissues like bones and teeth?
Celloidin embedding.
What is the main disadvantage of using the wet celloidin method?
It requires long processing time.
What is the advantage of using paraplast instead of paraffin wax?
Allows for better serial sectioning and is more elastic.
What is the function of plastic resins in embedding?
Used for light microscopy, especially in hard tissues like bone.
Which substitute for paraffin wax is used for enzyme histochemistry?
Carbowax.
Why should paraffin wax not be overheated?
It causes tissue shrinkage.
What is the best embedding method for delicate specimens and frozen tissue sections?
Gelatin embedding.
Why is the dry celloidin method preferred for whole eye sections?
It prevents tissue distortion and maintains transparency.
What is the main disadvantage of plastic resin embedding?
It is highly toxic and expensive.
What is the primary purpose of embedding?
To provide support for sectioning.
Which embedding medium is used in the double-embedding method?
Paraffin wax and celloidin.
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