Impregnation / Embedding

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44 Terms

1

What is the main purpose of impregnation in histology?

To replace the clearing agent with a medium that provides a firm consistency.

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2

Which of the following is NOT a type of tissue impregnation and embedding medium?

Acetone.

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3

What is the most commonly used infiltrating and embedding medium in histology?

Paraffin wax.

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4

Which of the following is an advantage of paraffin wax impregnation?

Allows rapid preparation of sections within 24 hours.

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5

What is the recommended immersion time for tissues in paraffin wax to prevent shrinkage and hardening?

Not more than 4 hours.

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6

Which of the following is NOT a method of paraffin wax impregnation?

Electrochemical processing.

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7

What is the function of the vacuum embedding method?

To speed up infiltration by removing air bubbles and clearing agents.

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8

In manual processing, how many changes of paraffin wax are required for proper impregnation?

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9

Which automatic processing machine is commonly used in tissue impregnation?

Autotechnicon.

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10

What is the routine melting point of paraffin wax used in histology?

56°C.

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11

Which substitute for paraffin wax is commonly used for embedding neurological tissues and hard tissues like bones and teeth?

Celloidin.

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12

What is the primary disadvantage of celloidin embedding?

Takes a long time for impregnation (several days to weeks).

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13

Why is gelatin impregnation rarely used?

It is water-soluble and difficult to section.

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14

Which embedding method is best for whole eye sections?

Dry celloidin method.

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15

What is the purpose of embedding in histology?

To provide a support medium for sectioning.

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16

What is the primary precaution to take when using vacuum embedding?

Avoid overheating to prevent tissue shrinkage.

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17

Which embedding mold is adjustable and allows different tissue sizes?

Leuckhart’s Embedding Mold.

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18

What is the primary advantage of the Tissue-Tek system?

Provides rapid embedding with easy orientation.

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19

Which substitute for paraffin wax is commonly used for embedding undecalcified bones?

Methyl Methacrylate (MMA).

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20

Which of the following describes the double-embedding method?

Uses a combination of celloidin and paraffin embedding.

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21

What happens if tissue is inadequately infiltrated during impregnation?

It retains the clearing agent and remains soft.

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22

Which of the following is the best embedding medium for routine histological procedures?

Paraffin wax.

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23

Which clearing agent is easiest to remove during paraffin impregnation?

Benzene.

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24

Which of the following is a disadvantage of paraffin wax embedding?

Cannot be used for enzyme histochemistry.

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25

What is the recommended embedding method for fatty tissues?

Water-soluble wax.

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26

What is the ideal melting point of paraffin wax used in histology when the lab temperature is 20-24°C?

54-58°C.

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27

How many wax changes are required in manual processing for complete impregnation?

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28

What is the function of the vacuum embedding technique?

To speed up impregnation and remove air bubbles.

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29

Which embedding medium is recommended for processing whole eye sections?

Celloidin.

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30

What is the purpose of using a cold plate during embedding?

To solidify the embedding medium rapidly.

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31

Which processing method uses an automatic tissue processor?

Autotechnicon processing.

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32

Which of the following is an advantage of the Tissue-Tek system?

Faster embedding and easier tissue orientation.

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33

Which embedding mold consists of two L-shaped strips of heavy brass?

Leuckhart’s embedding mold.

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34

Which embedding method is best for hard tissues like bones and teeth?

Celloidin embedding.

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35

What is the main disadvantage of using the wet celloidin method?

It requires long processing time.

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36

What is the advantage of using paraplast instead of paraffin wax?

Allows for better serial sectioning and is more elastic.

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37

What is the function of plastic resins in embedding?

Used for light microscopy, especially in hard tissues like bone.

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38

Which substitute for paraffin wax is used for enzyme histochemistry?

Carbowax.

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39

Why should paraffin wax not be overheated?

It causes tissue shrinkage.

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40

What is the best embedding method for delicate specimens and frozen tissue sections?

Gelatin embedding.

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41

Why is the dry celloidin method preferred for whole eye sections?

It prevents tissue distortion and maintains transparency.

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42

What is the main disadvantage of plastic resin embedding?

It is highly toxic and expensive.

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43

What is the primary purpose of embedding?

To provide support for sectioning.

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44

Which embedding medium is used in the double-embedding method?

Paraffin wax and celloidin.

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