Lecutre #10: factors that affect bacterial growth

List the 2 direct measurments of mircobiral growth:

total cell counts and viable counts

What is the one indirect measurement when measuring microbrial growth?

turbidometric methods

what is the name of the tool used to count microorganisms directly in a sample?

Petroff-Hauser Counting Chamber

True or False: Using the “direct microscopic count method” counts both viable(live) and non-viable(dead) cells.

True 

what type of dye is used for differentiation within direct cell counts?

methylene blue

What are the 2 main plate methods to viably count the number of living bacteria?

Spread-plate method and pour-plate method

what type of diltution method helps count bacterial cultures that are too concentrated?

serial dilutions 

What are the 3 sources of error in plate counting?

unsuitable culture conditions, procedural errors, and cell clumping

What are 4 environmental factos that affect microbial growth?

temperature, pH, water availabilty, and oxygen concentrations 

What are the three categories of growth within cardinal temperatures?

minimum, optimum, and maximum growth

Psychrophillic organisms have maximum growth temperatures below _______, optimal growth temperatures below ______, and minimum growth temperatures of ______ or lower.

20°C, 15°C, and 0°C

Psychrotolerant organisms can grow at ______, but have optimal growth temperatures of _________. They are found in temperate climates.

0°C; 20-40°C

___________ syringae in nature has a naturally occuring protein on its surface that acts as an ice-nucleation site, causing frost damage, usually on strawberries.However, Scientisits have made a syringe that removes the ice-nucleating protein.(helping them protect plants from frost damage). 

Psuedomonas 

Thermophiles have growth optima over _____

45°C

Hyperthermophiles have growth optima over _______.

80°C

True or False: Within thermophiles, eukaryotes have higher temperature growth abilites than prokaryotes.

False; prokaryotes do 

Does the bacterial or archeal branch contain the most thermopohilic species?

archael

Non-phototrophs can exceed phototrpahs in ________ growth abilites.

thermophillic