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Basic pieces of equipment in venipuncture
Gloves, tourniquets, alcohol swabs, gauze, tape, ETS, blood collection tubes, needles,sharp’s container, and requisition form
What is the standard needle gauge for venipuncture and what color is it
21 gauge; green
What needle is used for pediatric or small veins and what color is it
Blue; 23 gauge (often a butterfly)
What color or 22 gauge needles and what are the used for
Black; smaller veins
What is the needle stick safety and prevention act(2000)
A law aimed at reducing needlestick injuries among healthcare workers by promoting safer needle devices and practices.
Order of draw
Yes Little Bunny Rabbits Get Grabbed during Long Running Games
Order of Draw part. 2
Yellow, Light Blue, Red, Gold, Green, Lavender, Gray
What is the additive in yellow tubes
Sps
What is sps
Sodium polyanethol sulfonate, inhibits complement and phagocytosis
Common test for yellow tubes
Blood culture for microbiology
What do yellow tubes test on
Whole blood
What is the additives in light blue tubes
Sodium citrate
What does sodium citrate do
It’s an anticoagulant that preserves clotting factors
What is a common test for light blue tubes
Coagulation tests, PT,PTT, INR, D-dimer
What does the light blue tube test on
Plasma
what are the additives in red tubes
None(glass) OR silica clot activators(plastic)
addative that stimulate clotting
Clot activator
What are common test for red tubes
chemistry, serology, Blood bank
What is in gold/tiger top tubed
Serum separator gel or polymer gel
What is polymer gel/ Thixotropic gel
forms a separation barrier between serum and cells after centrifugation
What are common test done by gold/tiger tops
Tests for serum chemistry, such as lipid panel, liver function tests, and thyroid tests, do most infectious disease tests
What is SST
serum separator tube, contains a gel that separates the serum from the blood cells after centrifugation
What is in dark green tubes
sodium heparin; an anticoagulant for plasma collection or Lithium heparin
What are common dark green tube tests
Arterial blood gas (ABGs), Electrolytes, ammonia
What is in lavender tubes
EDTA, an anticoagulant used to prevent blood clotting.
What are common tests for lavender tubes
complete blood counts (CBC), ESR (Erythrocyte Sedimentation Rate)
what is EDTA
ethylenediaminetetraacetic acid; binds calcium to stop coagulation cascade
What is in gray tubes
sodium fluoride and potassium oxalate
What are common tests for Grey tubes
Glucose testing, lactate acid testing, and blood alcohol levels.
what do gray tubes test on
plasma or serum
Median cubital vein
The primary vein used for venipuncture, located in the middle of the antecubital fossa. It is preferred due to its size and accessibility.
Cephalic vein
A vein in the arm that runs along the thumb side and is often used for venipuncture.
Basilic vein
A vein located on the inner side of the arm that is generally more challenging to access but can be used for venipuncture.
agent that stop bacteria growth
Antiseptic
Hollow tube within the needle
lumen
Chemical added to evacuated tubes
to preserve or enhance the quality of the blood sample during collection or Additives
metabolic sugar breakdown in cells
glycolysis
What is Hemolysis
The breakdown of red blood cells
iatrogenic anemia
Anemia caused by excessive medical treatment or procedures
what is bacteriostatic
A solution that inhibits the growth of bacteria but does not kill them; to prevent bacterial contamination.
difference between outpatient and in patient
Outpatients receive care without being admitted to a hospital, while inpatients are admitted for at least one night for treatment.
what is heparin
an anticoagulant used to prevent the formation of blood clots by inhibiting thrombin and factor Xa in the coagulation pathway.
difference between sodium heparin and lithium heparin
Sodium heparin is used for blood tests and as an anticoagulant, while lithium heparin is often used in specific tests requiring minimal interference.
what is Inversion and how many times should it occur
gentle mixing blood collection tubes for proper mixing of the anticoagulant with the blood sample: 5-10 times depending on the tube type.
What is PST
Plasma Separator Tube, which contains gel and anticoagulant to separate plasma from blood cells during centrifugation.
relationship between needle luman and gauge
inverse; as the gauge number increases, the needle's lumen diameter decreases. This means larger gauge needles have thinner walls and smaller openings.
Luer adaptor
allows secure connection between a syringe and a tube or needlesmooth transfer of fluids.
antiseptic vs disinfectant
Antiseptics reduce the risk of infection on living tissues, disinfectants are used to destroy bacteria and viruses on inanimate surfaces.
16-18 inch Guage
used for blood bank/ donation
difference between red glass tubes and red plastic tubes
Red glass tubes are used for serum collection and dont contain additives, while red plastic tubes contain a clot activator, allowing for quicker clot formation.
thrombin
A blood-clotting agent used in laboratory settings, often found in clot activator tubes.
difference between anticoagulants and clot activators
Anticoagulants inhibit blood clotting, while clot activators promote coagulation for quicker serum separation.
what do red tube test on
Serum
what gold/tiger tubes test on
serum
what do green tubes test on
plasma
what do lavender tubes test on
whole blood
difference between whole blood, plasma, and serum
Whole blood has all components of blood, plasma is the liquid portion after centrifugation without clotting, and serum is the liquid left after coagulation has occurred.