Lab 1 basic skills

Serogical Pipette

• The pipette pump can be automatic (electric) or manual 

Pasteur Pipette

• Will have a bulb at the top of the pipette 

Micropipette

• Has a disposable tip and variable volume pipettors 

Serological Pipette 

Pasteur Pipette 

• Pipette is disposable in DC Gold

• Bulbs at the top are reusable 

• Used to transfer liquid, not measure liquid 

• Usually only holds 1-2 ml of liquid 

Pasteur Pipette 

• Pipette is disposable in DC Gold

• Bulbs at the top are reusable 

• Used to transfer liquid, not measure liquid 

• Usually only holds 1-2 ml of liquid 

Micropipettes

• AKA variable volume pipettors 

• Measure small volumes: only up to 1 ml (or 1000 ul) and are the most accurate of all pipettes 

• Use disposable tips 

  • Different size tips for different size pipettes 

  • ONE TIME USE (usually) and disposable in biohazard bin

How to use a micropipette

• NEVER point up 

• Use the smallest pipette possible without exceeding your desired amount

Example: 

If you need 3ul, use a P10 micropipette. 

If you need 99 uL, use the P100, but if you need 100 uL, use the P200

what will happen if u exceed the amount the pipette is made for

Tips for using the micropipettes 

• Tip should be directly vertical and about 1 cm into the water

Tips For Using the Micropipettes 

To aspire liquid:

• You should be at the first stop position before entering the liquid 

• Enter tip halfway into the liquid 

• Release plunge to obtain liquid

To dispense liquid:

• Set tip into the dispense vessel

• Slowly push plunger to the first stop

• Raise tip slightly above the dispensed liquid, then push to the second stop 

• While still on the second stop, pull whole tip out of liquid 

• Now, you can release the plunge and dispose of your tip