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CC
Chief Complaint
Segment
Line between two waveforms
P Wave
Arterial Depolarization
How many 1mm boxes are in a 6 second rhythm strip?
30 one millimeter boxes
The SA node represents which waveform on the EKG?
Only the P wave
What is the reading you can get from the RR interval?
Ventricular Pulse
What is the primary stage of Hemolysis?
Vascular phase and platelet phase together
What is Electrophoresis?
Analyzation of the chemical components of blood, e.g. hemoglobin, serum, urine, cerebrospinal fluid based on electrical charge.
Positive testing for occult blood in a stool sample turns which color?
Turns blue on a positive test for occult blood in feces
The stages of Hemostasis
1. Vascular, 2. Platelet Phase, 3. Coagulation Phase,
4. Fibronolysis
Blood vessels
Aorta, Arteries, Arterioles, Capillaries, Venues, Veins, Superior and Inferior Vena Cavae
Outer connective tissue layer of a blood vessel
Tunica Adventitia
Inner endothelial tissue of a blood vessel
Tunica Intima
The inner smooth layer of a blood vessel
Tunica tunica media
The standards of right and wrong in a medical setting
Code of Ethics
What do you find in capillaries?
A mixture of venous and arterial blood is found
The average adult has how many liters of blood?
5-6 Liters
What is the percentage of water in the blood?
92%
Percentage of plasma in the blood?
55%
Percentage of formed elements in the blood?
45%
99% of formed elements in the blood
RBC,s or Erythrocytes
Where do you find hemoglobin?
RBC,s
Where do all blood cells originate?
Bone marrow
How many RBC,s per microliter of blood
4.2-6.2 million
What is the second most numerous WBC
Lymphocyte with 20-40%
Their numbers increase in intracellular infections and TB
Monocytes
Their numbers increase in bacterial infections and are often first on the scene
Neutrophils
These WBC,s play an important role in viral infection as well as in immunity
Lymphocytes
Number of leukocytes for average adult per microliter
5000-10000
Leukopenia
Decrease in WBC,s seen with viral infection and leukemia
Comprises 3-8% of WBC or leukocytes
Monocytes
Largest in size of the leukocytes
Monocytes
Carries histamine
Basophil
Injury to a blood vessel that causes it to constrict, slowing the clod of blood.
Vascular phase of Hemostasis
Preferred site for a venipuncture
Antecubital Fossa
Test used to evaluate the intrinsic pathway and monitor heparin therapy
APTT also know as PTT
Test used to evaluate the extrinsic pathway and also used to monitor warfarin therapy
PT
Fibrinolysis
Breakdown and removal of a clot
Converts the temporary platelet plug in a stable fibrin clot
Coagulation Phase
2nd choice vein for venipuncture and most often the only one palatable in an obese Pt.
Cephalic vein
Antiseptics used in Phlebotomy
70% isopropyl alcohol pads most common, provolone - iodine for BC,s and Chlorhexidine gluconate for patients that are allergic to Betadine/ Iodine
Length of needle commonly used in venipuncture
1 inch (up to 1.5")
Average gauge of needle used for drawing blood
21-22
Gauge of needle that can cause hemolysis
smaller than 23 gauge
3 skills of the phlebotomist
Social, clerical, technical
Analytical errors during collection of blood
Extended tourniquet time, hemolysis, wrong order of draw, failure to invert tubes, faulty technique under filling tubes
Analytical errors before collection of blood
Patient misidentification, improper time, wrong tubes, not fasting, exercise (Cortisol levels), Pt. Posture, Poor coordination with other treatments, improper site prep, medication interference
Analytical errors After collection of blood
Failure to separate serum from cell (Glycolisis), improper use of serum separator (Not inverting tubes or not spinning tubes), delays in processing, exposure to light, improper storage conditions, dimming clots
Fistula
Permanent surgical connection between an artery and a vein, never used for venipuncture
Edima
Accumulation of fluid in tissue
Thrombophlebitis
Inflammation of a blood clot formation
Explanation of Hemoconcentration
The increase in proportion of formed elements to plasma caused by leaving on the tourniquet for more than two minutes
Consequence of insufficient pressure applied after withdrawal of a needle
Thrombus
Additives in a green top tube
Heparin, sodium, lithium, ammonium
Why is the green top tube never used for hematology?
Green tops additive heparin interferes with the Wright's stained blood test
Common tests for the lavender EDTA tube
CBC, differential or diff, ESR, sickle cell screening
Common tests for this color tube are Chemistry tests performed on plasma such as ammonia, carboxyhemoglobin and STAT electrolytes
Green top tube (Heparin) tests
How long does it take for blood to clot by the normal coagulation process in the red top tube
30-60 mins
The primary purpose is to provide reliable data about a patients health status by ensuring the accuracy of a test while detecting and eliminating error
What is Quality Control?
Common test for the red topped tube
Serum Chemistry tests, serology tests, blood bank (Glass only)
Order of the draw for capillary specimens
Lavender first, than tubes with other additives, than tubes without additives
Antiseptic not used on a dermal puncture site
Betadine (Because it interferes with several tests like bilirubin, uric acid, phosphorus, and postassim)
For which procedure would you warm the site for a minimum of 3-4 minutes to increase blood flow?
Dermal punctures (Heels sticks)
Which are the preferred sites for dermal punctures?
The distal segment of the third or fourth finger of the non-dominant hand
Heel sticks are performed on which patients?
Infants less than 1 years old
Where on the foot is the dermal puncture made on patients less than 1 years old?
The medial and lateral areas of the plantar surface of the foot
Dermal puncture is made in
The fleshy portion of the finger slightly to the side of the center perpendicular to the lines of the fingerprints
what will occur if you puncture the heel too deep? More than 2mm
Osteomyalitis
what are the identification requirements for the blood bank?
Patients full name or DOB, Hospital ID# or SNN for outpatient care, date and time of collection as well as the phlebotomist's initials
What does the toxicology section of the lab analyze?
Plasma levels of drugs and poisons
What is the percentage of the population with the D antigen present?
85% of the population
The 4 blood types
A, B, AB, O
Which blood type has neither the Anti-A nor the Anti-B plasma antibodies
AB
This section of the lab uses serum to analyze the presence of antibodies to bacteria, fungi, viruses, parasites, and antibodies against the body's own substances?
Serology (Immunology) Section
Mycology
Study of fungi
The physical examination of urine consists of
Color, Clarity, Specific gravity
A low power microscope has a magnifying power of
100X
Oil immersion objectives on the microscope reach a maximal magnification of
1000X
This objective of the microscope is used for observing bacteria, WBC differential count and RBC morphology
Oil immersion
Eyepiece of the microscope
Ocular lens
Focal length is?
The distance of the object to be examined to the center of the lens
Meter to inches
39.37
High power objective on a microscope magnification
400X
1000 meter are equal to
1km
1mm to inches
0.04
0.3 meter of tubing is how many inches
12 inches
1kg is how many pounds
2.2 pounds
Abbreviation of microliter
ul
Abbreviation for decimeter
dm
Gram stain positive stains
Deep violet (Violet to black)
Gram stain negative stains which color
Light to dark red
Bacteria that take up and retain the crystal violet and resist alcohol discoloration
Gram positive bacteria, appear blue to black
Bacteria that are decolorized completely by ethanol and take safrin counterstain
Gram negative bacteria, appear red
Gram stain sequence
Crystal violet, Gram's iodine, 95% ethyl alcohol/acetone mix/safranin stain
The degree of angle of the pusher slide
30 degrees
The urinary system
Two kidneys, two ureters, bladder, urethra
When checking for hormones in urine when do you collect the urine sample?
First voiding in the morning
Which urine specimen provides the clearest, most accurate results?
Clean catch midstream specimen
Which urine sample requires aseptic technique
Clean catch specimen
Examination of urine consists of
Physical, chemical, microscopic