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What defines Clade Ecdysozoa?
A group of 8 animal phyla defined by molecular phylogeny; all periodically shed a cuticle via ecdysis.
What are the two largest ecdysozoan phyla?
Nematoda (most numerous) and Arthropoda (most biodiverse).
What is the cuticle in ecdysozoans?
A tough, non-living, organic outer layer secreted outside the epidermis.
What are the functions of the cuticle?
Protection from desiccation/injury, body shape, muscle attachment.
What is the cuticle composed of?
Chitin and proteins; has three layers: endocuticle, exocuticle, and epicuticle.
What is chitin?
A long-chain nitrogenous polysaccharide found in the cuticle.
What is the endocuticle?
The innermost layer of the cuticle; made of chitin and procuticle.
What is the exocuticle?
The middle layer of the cuticle; made of chitin and procuticle.
What is the epicuticle?
The thin, waxy, outermost layer of the cuticle.
Why must ecdysozoans moult?
The cuticle can't grow; moulting (ecdysis) allows size increase and stage changes.
What is sclerotization?
Hardening of the cuticle via cross-linking chitin with proteins.
What are sclerites?
Rigid cuticle plates formed by sclerotization.These plates provide structural support and protection to the organism.
What is apolysis?
The separation of the old cuticle from the epidermis (first step of moulting).
Describe the sequence of ecdysis.
Apolysis
Inactive moulting fluid secreted
Epidermis grows (folds)
Proto-epicuticle deposited
Enzymes activate, digest old endocuticle
New procuticle deposited
Moulting fluid reabsorbed
New epicuticle completed
Old exocuticle/epicuticle shed
New procuticle sclerotized → becomes new endo/exocuticle
Where are nematodes found and what roles do they play?
Moist environments; free-living predators/microbivores or parasites.
Key features of nematode body plan?
Unsegmented, no appendages, complete digestive tract, hemocoel.
What is Caenorhabditis elegans?
A model nematode species widely used in biological research.