[Elsevier] Microbiology - Aerobic Gram positive bacilli (for review)

All aerobic gram (+) bacilli grow easily after 24 hours on what agar?

SBA

Catalase (+) spore forming gram (+) aerobic bacilli

Bacillus spp.

Non-spore forming gram (+) aerobic bacilli

Listeria, Erysipelothrix, Corynebacterium

Unique formation of Bacillus spp.

Endospores

Most common disease manifestation of this bacteria is food poisoning

Bacillus cereus

Food poisoning of B. cereus via meat; lasts 24 hours

Diarrheal

Food poisoning of B. cereus via fried rice; lasts 10 hours

Emetic

The colonies of this bacteria are:
Large rods, spreading
Beta-hemolytic
Catalase (+)

Bacillus cereus

It is the most virulent and significant human disease

Anthracis

Three clinical presentations of anthracis

Cutaneous, Inhalation, Gastrointestinal

MOT of Cutaneous anthracis

Direct contact

MOT of wool sorters disease

Aspiration of spore aerosol

MOT of GI anthracis

Eating of contaminated meat

Inhaled B. antrhacis presents itself as what disease

Wool sorters disease

Most common form of anthracis; has an incubation period of ____ days.

Cutaneous, 1 to 12 days

Which type/s of anthracis is/are localized? Which type/s of anthracis is/are septic?

Cutaneous is localized; Inhalation and GI are septic

The colonies of this bacteria are:
Medium to Large irregular rods, gray
Medusa head projections
Non-hemolytic
Catalase positive

Bacillus anthracis

These bacterial spp. appear as gram (+) short rods or coccobacilli, they are non-acid fast and are able to grow at 4 degrees celcius

Listeria spp.

The colonies of this bacteria are:
Gram (+) coccobacilli
Beta-hemolytic
Can grow at 4 degrees celcius
Umbrella motility

Listeria monocytogenes

Listeria monocytogenes has a characteristic...

Umbrella motility

Bacillus anthracis has a characteristic...

Medusa head protrusion

These bacteria are non-spore forming gram positive rods closely related to Listeria spp.
They occur in short chains or filaments, and can be facultative anaerobes
They are nonmotile
Catalase negative

Erysipelothrix

Causative agent of erysipelas in pigs and a cuteneous infection known as erysipeloid in humans (MOT: Direct contact with infected animals)

Erysipelothrix rhusiopathiae

The colonies of this bacteria are:
Cultured from a skin biopsy
Pleomorphic gram (+) rods
Cultured on BAP with 5-10%CO2
H2S (+) on Triple Sugar Iron (TSI) slant

Erysipelothrix rhusiopathiae

Cutaneous anthracis begins as a ____, progresses to a ____, then depresses into a ____.

Papule, vesicular stage, eschar

These bacteria are small dry gram (+) bacilli, commonly refered to as coryneforms due to their clublike morphology; they may also resemble chinese letters

Corynebacterium spp.

This bacterium is acquired by person to person contact with carriers; it typically manifests itself as respiratory infections to airway obstruction, kidney and liver inflammation, myocarditis, but may also have a cutaneous infection
Disease hallmark are adherent membranes of the tonsils, pharynx or nose

Corynebacterium diptheriae

Hallmarks of C. diptheriae are adherent membranes of the _____.

Tonsils, pharynx, and nose

Proliferation of C. diptheriae in tissues causes

Pseudomembrane formation

How does C. diptheriae cause death of eukaryotic cells?

Protein synthesis inhibition

The colonies of this bacteria are:
Cultured from throat and nasopharynx
Black to gray in CTBA (Cystine-tellurite medium)
Black with brown halos in Tinsdale medium
Gram (+)
Urease (-)
Nitrate (+)
Catalase (+)

Corynebacterium diptheriae

Selective medium for C. diptheriae; the bacteria appear as _____ in this medium

Cystine-tellurite medium (CTBA), black to gray

Differential medium for C. diptheriae; the bacteria appear as _____ in this medium

Tinsdale medium, black with brown halos

In vivo culture of C. diptheriae is

Culture and antitoxin in rabbits