Microscopy and Microscopic staining

microscope helps..

helps visualize samples since they are very small microorganisms

Purpose of microscope

magnify image you are looking at; works by light traveling in straight line until it hits reflective surfaces

ultimate response of microscope

combination of light shinning through a very thin specimen on the slide + coming in contact with reflective surface of microscopes in lab to enhance image of microorganism(provides more details)

Resolution

the ability to identify two organisms that are a certain distance apart as separate entities

parts of compound microscope

ocular lens, body, arm, objective lens, stage condenser, diaphragm, illuminator, coarse focusing know, fine focusing knob, base

light path of compound microscope

travels up from illuminator; in ocular lens it reflects image; inverse + upside down

Types of light microscopy

compound light microscope and dissecting scope

types of images we see from light microscopy

bright field, dark field, phase( phase-contrast and differential interference), fluorescent, confocal

Light microscopy

very diverse; can make small adjustments + change type of image you see

Bright field

unstained specimen, uses diaphragm to maximize light source that hits specimen, gives bright background + specimen appears dark; not many details, only general info

dark field

unstained, negative staining, darker background + unstained cell becomes light, restrict light by changing condenser settings, adding black dye

phase-contrast

alters wave-length of light that hit specimen, exposing specimen to different wavelengths of light(allows 3D image) greater resolution of internal surfaces

differential interference

3D surface images, increases contrast

Fluorescent

allows to target specific proteins(indemnifies specific protein), involves lab work manufactured antibodies in lab with specific protein other side added artificially compound that glow when binded