Serial dilution
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10 Terms
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Serial Dilution
A stepwise dilution of a substance in solution, used to prepare solutions of lower concentrations in chemistry and biology.
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Concentration
The amount of solute in a given volume of solvent.
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Dilution Factor (DF)
The ratio of the initial concentration to the final concentration.
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Total Dilution
The overall dilution achieved after multiple steps.
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Final Concentration (C_f)
The concentration after dilution, calculated using the initial concentration and the dilution factor.
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Steps to Calculate Serial Dilution
1. Determine the Initial Concentration (C_i). 2. Decide the Volume of the Final Solution (V_f). 3. Choose the Dilution Factor (DF) for each step. 4. Add the appropriate volume of Diluent (solvent). 5. Mix Well.
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Formula for Dilution Factor (DF)
DF = V_initial / V_final.
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Formula for Final Concentration (C_f)
C_f = C_i / DF.
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Important Note on Dilution Factors
The Total Dilution is the product of individual dilution factors.
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Bacteria Calculation in Serial Dilution
To find the total amount of bacteria, multiply the concentration by the dilution factor after determining the number of bacteria at each step.
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