Host Basics-Arthrobacter sp.

Arthrobacter sp.

A gram-positive soil organism that is widely used as a host bacterium for phage isolation in SEA-PHAGES.

Actinobacteria

A phylum of bacteria that includes Arthrobacter sp. and other related species, known for their ability to breakdown various hydrocarbons.

Bioremediation

The process of using organisms, such as Arthrobacter sp., to breakdown pollutants and remove them from the environment.

Colony

A visible cluster of cells that arise from a single bacterium, typically takes about 3 days for an individual cell of Arthrobacter sp. to form a colony on an agar plate.

Growth media: PYCa

The specific nutrient-rich media used to grow Arthrobacter sp. in SEA-PHAGES research.

Antimicrobials: cycloheximide (10 μg/ml), spectinomycin (optional)

Antifungal and antibiotic substances that can be added to the growth media to prevent the growth of other microorganisms in Arthrobacter sp. cultures.

Phage Buffer

A solution containing Tris, MgSO4, NaCl, and CaCl2, used in phage isolation experiments with Arthrobacter sp.

Restriction Enzymes: BamHI, ClaI, EcoRI, HaeIII, HindIII, MseI

Enzymes used to cut DNA at specific recognition sites, which are commonly used in genetic engineering experiments involving Arthrobacter sp.

Isoschizomers

Different restriction enzymes that recognize the same DNA sequence, which can be used as alternatives to the listed restriction enzymes for Arthrobacter sp. experiments.