Microbiology For Allied Health Final Study Guide Unit 1 Test

According to the resistance table, which microorganisms seems to carry the least resistance?

Lipid envelope viruses

If you grow two species of bacteria on a medium to determine what type of organisms they are and what their fermentation pattern looks like, which type of medium do you use?

Both differential and selective medium

What is the most frequently used method of counting bacteria?

Serial dilution and viable plate count

Alcohols denature proteins in the cell membrane of microbes, what is the ideal concentration of alcohol as a disinfectant?

50%

Most of the available antimicrobial agents are effective against

Bacteria

What is the method of communication microbes that live in a biofilm use

Quorum sensing

More than half of our antibiotics are

produced by bacteria

Salts and sugars work to preserve foods by creating a

High osmotic pressure

Handwashing technique was first proposed by

Ignaz Semmelweis

Which of the following antimicrobial agents is recommended for the use against fungal infections?

Imidazole

During which phase would penicillin, an antibiotic that inhibits cell-wall synthesis, be most effective?

Log phase

What is the disadvantage of Petroff-House cell counter?

It counts live and dead cells

All viruses are lipid enveloped viruses

False

Medications used to treat viruses are called antibiotic.

False

During the stationary phase of the bacterial growth curve, ______

The organisms are dying just as quickly as they are multiplying.

Which of the following microorganisms is considered the most resistant?

Prions

Microbes are found in which habitat

Human body, Earth's crust, Oceans

Chemicals used to remove bacteria from inanimate objects are called _______

Disinfectant

Which of the following theory states that all cells come from preexisting cells?

Cell Theory

Which of the following microorganisms is considered the lease resistant?

Lipid envelope viruses

This type of microbial control can lead to neurological damage in infants.

Hexachlorophene

Which of the following actions would be the most clearly considered bacteriostatic rather than bacteriocidal?

Placing bacterial culture in the -80 C freezer

Which of the following is typically found in mouthwash?

Cetyl Pyridinium Chloride

To date, most of our natural antibiotics have been found to be produced by members of what genus?

Streptomyces spp.

What is the goal number of CFUs on a spread plate if a serial dilution is done correctly?

30-300 per plate

The Germ Theory was first proposed by

Louis Pasteur

What is the main purpose of a streak plate?

To obtain pure cultures

Christian Hans Gram developed _____

Gram stain technique

Nosocomial infections are infections that ______

Are acquired in hospital and/or clinical settings

"EMB is a medium used in the identification and isolation of pathogenic bacteria. It contains digested meat proteins, soytone and peptone as a source of organic nutrients. Two indicator dyes, eosin and methylene blue, inhibit the growth of gram-positive bacteria and distinguish between lactose fermenting and nonlactose fermenting organisms. The media also contains 15g of agar per litter. Lactose fermenters form metallic green or deep purple colonies, whereas the nonlactose fermenters form completely colorless colonies. EMB agar is an example of which of the following?"

A solid media that is complex, selective and differential.

Chemicals used to remove bacteria from living tissues are called _______

Antiseptics

One gram of a soil sample was added in a 9 ml sterile water (Mix A). 0.1 ml of Mix A was added in a 9.9ml of sterile water (Mix B). 2ml of Mix B was added in a 18 ml of sterile water (Mix C). How much was the soil sample diluted after mix C? o.1 ml of Mix C was plated on a agar plate. After incubation 325 colonies were counted. Calculate the CFU/g of the sample.

We can't calculate the CFU because the number of colonies counted was above 300.

Cell theory stated that living organisms must contain cells and cells can originate from anything.

False

Which of the following statements is true?

Louis Pasteur disproved Spontaneous Generation

Which of the following is not a mode of action of disinfectants?

All of the above are considered mode of action of disinfectants

Liquid growth media that contain antibiotics should be sterilized using which method?

Filtration

Which of the following is (are) not considered as Properties of Life

All living organisms contain a chloroplast

Which best describes bacterial growth during the log phase of the growth curve?

Exponential

The invention of the microscope was credited to ______

Antwon Van Leeuwenhoek

This group of microorganisms is responsible for the majority of foodborne outbreaks.

Viruses

Which of the following is an acellular microorganism?

Virus

Based on the result of this Kirby Bauer test below, the most effective antibiotic is Antibiotic_________

A (One with the biggest ZOI)

Food spoilage is often caused by which type of microbe?

Psychrotrophs

"This string of DNA, 3 ATCGTTTAGCATTA 5 , would be most likely affected by which method of microbial growth control?"

UV Light

The addition of which of the following to a culture will neutralize acids?

Buffers

___________ introduced phenol as the first disinfectant for surgical instruments

Joseph Lister

The concept of Biogenesis was first introduced by _____

Robert Hooke

"During the exponential phase of the bacteria growth curve, __________"

It is the best of time to test organisms because they are metabolically active.

This substance is incorporated into cutting boards in an attempt to control microbial growth.

Triclosan

Transient flora are opportunitist pathogens

True