MICROBIO 4.2

0.0(0)
Studied by 0 people
call kaiCall Kai
Locked
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/14

flashcard set

Earn XP

Description and Tags

AGAR PLATES

Last updated 3:57 AM on 8/12/26
Name
Mastery
Learn
Test
Matching
Spaced
Call with Kai
Chat

No analytics yet

Send a link to your students to track their progress

15 Terms

1
New cards

LB Agar

Non-selective, non-differential

Grows E.coli for molecular biology applications

<p>Non-selective, non-differential</p><p>Grows E.coli for molecular biology applications</p>
2
New cards

Trypticase Soy Agar (TSA or TSAYE)

Non-selective, non-differential

Serves as base for enriched medias

<p>Non-selective, non-differential</p><p>Serves as base for enriched medias</p>
3
New cards

Blood Agar (BAP)

Non-selective, differential, enriched

Grows fastidious microorganisms

Differential: Detects hemolytic activity

Differentiates Streptococcus strains

<p>Non-selective, differential, enriched</p><p>Grows fastidious microorganisms</p><p>Differential: Detects hemolytic activity</p><p>Differentiates Streptococcus strains</p>
4
New cards

Hemolysis

Breakdown of RBC

5
New cards

Alpha hemolysis

Partial lysis of RBC; greenish-brown color colonies

<p>Partial lysis of RBC; greenish-brown color colonies</p>
6
New cards

Beta hemolysis

Complete lysis of RBC; distinct zone of clearing around colony

<p>Complete lysis of RBC; distinct zone of clearing around colony</p>
7
New cards

Gamma hemolysis

No lysis

<p>No lysis</p>
8
New cards

Chocolate Agar (CHOC or CBA)

Non-selective, non-differential, enriched

Derivative of blood agar; made of already LYSED RBC (by heat)

Enriched: fastidious and pathogenic bacteria

<p>Non-selective, non-differential, enriched</p><p>Derivative of blood agar; made of already LYSED RBC (by heat)</p><p>Enriched: fastidious and pathogenic bacteria</p>
9
New cards

Colombia CNA Agar

Selective, differential, enriched

Selective: antimicrobial agents (colistin and nalidixic acid); FOR gram POSITIVE

Differential: blood —> hemolytic patterns

<p>Selective, differential, enriched</p><p>Selective: antimicrobial agents (colistin and nalidixic acid); FOR gram POSITIVE</p><p>Differential: blood —&gt; hemolytic patterns</p>
10
New cards

Mannitol Salt Agar (MSA)

Selective, differential

Selective: NaCl (7.5-10%); selective FOR gram POSITIVE

Differential: Mannitol & pH (phenol red); differentiates pathogenic strains of Staph

Pathogenic FERMENTS mannitol → agar Δ color from red (>8.2) to yellow (<6.8)

<p>Selective, differential</p><p>Selective: NaCl (7.5-10%); selective FOR gram POSITIVE</p><p>Differential: Mannitol &amp; pH (phenol red); differentiates pathogenic strains of Staph</p><p>Pathogenic FERMENTS mannitol → agar Δ color from red (&gt;8.2) to yellow (&lt;6.8)</p>
11
New cards

MacConkey Agar

Selective, differential

Selective: Crystal violet & bile salts RESTRICT gram POSITIVE

Differential: Lactose & pH; red means acidic, which means lactose is fermented

Colonies change color AND base (surrounding area) sometimes

pH > 8.0 → agar turns yellow; pH <6.8 → agar turns darker red or pink

Pale red

<p>Selective, differential</p><p>Selective: Crystal violet &amp; bile salts RESTRICT gram POSITIVE</p><p>Differential: Lactose &amp; pH; red means acidic, which means lactose is fermented</p><p> Colonies change color AND base (surrounding area) sometimes</p><p> pH &gt; 8.0 → agar turns yellow; pH &lt;6.8 → agar turns darker red or pink</p><p>Pale red</p>
12
New cards

Sorbitol-MacConkey Agar (SMAC)

Selective, differential

Differential: detects pathogenic strain of E. coli (O157:H7)

E. coli O157:H7 unable to ferment sorbitol → colorless/white colonies instead of red

Contains sorbitol instead of lactose

<p>Selective, differential</p><p>Differential: detects pathogenic strain of E. coli (O157:H7)</p><p>E. coli O157:H7 unable to ferment sorbitol → colorless/white colonies instead of red</p><p>Contains sorbitol instead of lactose</p>
13
New cards

Eosin Methylene Blue Agar (EMB)

Selective, differential

Selective: Eosin & methylene blue RESTRICT gram POSTIVE

Differential: Lactose;

Non-ferm → white/tan colonies; lactose-ferm → dark (purple to black) colonies

E. coli has metallic green sheen (a LOT of acid)

<p>Selective, differential</p><p>Selective: Eosin &amp; methylene blue RESTRICT gram POSTIVE</p><p>Differential: Lactose; </p><p> Non-ferm → white/tan colonies; lactose-ferm → dark (purple to black) colonies</p><p> E. coli has metallic green sheen (a LOT of acid)</p>
14
New cards

Agar

Polysaccharide made from algae/seaweed

Microbes can’t digest !

15
New cards

How to make agar

  1. Add powdered agar to water (or growth media)

  2. Heat to 100 degrees Celsius so it dissolves (100 degrees retains most of nutrients added to media & won’t damage antibiotics)

  3. Solidify at below 40 degrees Celsius (sensitive ingredients like blood can be added before agar solidifies)

  4. Solid until greater than 100 degrees again !