PROTOZOANS: INTESTINAL AMOEBA

protista

kingdom of protozoans

cilia, flagella, and pseudopodia

locomotory organs of protozoans

large intestine (except E. gingivalis)

Habitat of intestinal ameba

encystation

The process by which a trophozoite morphs into a cyst

E. gingivalis

All intestinal ameba undergo encystation except ___________________________

cyst (except E. gingivalis)

infective stage of all intestinal ameba

E. histolytica

All intestinal ameba are commensals except ________________________

Sexual, asexual or both

protozoans reproduce in what way

15-20 um

trophozoite size of E. histolytica

20-25 um

trophozoite size of E. coli

progressive, unidirectional

motility of E. histolytica

Non-progressive, sluggish

motility of E. coli

Finger-like Hyaline

E. histolytica pseudopodia

Blunt, Granular

E. coli pseudopodia

1

how many nuclei do E. entamoeba trophozoites have?

1

how many nuclei do E. coli trophozoites have?

fine, smooth, even

e. histo peripheral chromatin

coarse, rough, uneven

e. coli peripheral chromatin

centrally located; small

e histolytica karyosome

eccentric, large

e. coli karyosome

E. histolytica

clean looking with ingested RBCs are the cytoplasmic inclusions of ________________________

E. coli

dirty looking with ingested bacteria/debris are the cytoplasmic inclusions of ________________________

12-15 um

cyst size of E. histolytica

15-25 um

cyst size of E. coli

up to 4

how many nuclei do E. histolytica cysts have?

up to 8

how many nuclei do E. coli cysts have?

cigar shape/ sausage shaped

chromatoidal bar of e. histo

splintered or broomstick appearance

chromatoidal bar appearance of E. coli

chromatoidal bar

energy source of intestinal ameba (made up of RNA)

E. dispar, E. moshkovski (Laredo strain), and E. bangladeshi

Entamoeba species that have the same size and appearance similar to E. histolytica are:

not only differentiated by using microscopy, but also by means of molecular techniques, isoenzyme analysis & zymodeme analysis

How are E. dispar, E. moshkovski (Laredo strain), E. bangladeshi differentiate from E. histolytica?

F: 90% are asymptomatic, 10% are symptomatic

T or F: 10% of E histolytica infections are asymptomatic

amebic dysentery, flask shaped ulcers, colitis, and amebomas

symptomatic cases of infection causes these s/s:

"anchovy sauce aspirate"

amoebic liver abscesses have this characteristic:

#1 Lectin - used for attachment

#2 Amebapores- forms holes

#3 Cysteine proteases- for spreading/ damage of ameba

Virulence factors of E. histolytica

FECT

lab technique used torecover cyst stages

Robinson Inoki Medium

recommended media for cultivating ameba

metronidazole

treatment for amebiasis

diloxanide furoate

Treatment for asymptomatic carriers of amoebiasis.

E hartmanni

" Small race of Entamoeba histolytica"

Angular or pointe chromatoidal bars

distinct feature of E. pollecki

E. chattoni

E polecki is morphologically similar to:

WBCs

E. gingivalis is able to ingest _______________________

mouth scraping examination

diagnosis of E gingivalis is done with a:

large glycogen vacuole and "Basket of flowers" appearance of karyosome

distinct feature of I. butschlii

Lugol's iodine

I. butschli's large glycogen vacuole stains with:

brown; colorless

Iodamoeba butschlii is colored __________ in fresh wet mount, and __________________ in permanent stain

I. butschlii

absence of peripheral chromatin in intestinal ameba is only seen in ______________________

E. nana

"Cross-Eyed Cyst" with eccentric karyosome

E. gingivalis

N. fowleri

Trichomonas app.

Amoebas with trophozoite as infective stage: