Genetics module 10 Dna sequencing

what qualies do a terminator base have

lack an oh group

have a fluorescent marker

type of electrophoresis used with dna sequencing

capillary

automated sanger dna sequencing ingredients

template strand, pol, primer strand, dna polymerase with high fidelity, dntps, ddntps, buffer, magnesium, water

genomic dna library

set of dna clones collectively containing the entire genome of an organism

cdna library

contains the codign regions of the expressed genes of an organism, made of complementary dna (cdna) synthesized from rna by reverse transcriptase

what 3 features make mrna an attractive template

have already been spliced, have a poly a tail that a poly t primer can be attached to, has only relevant and functional information

what enzyme is used in the first strand sequence

reverse transcriptase

what primer is used and is it specific

oligot - specific

rnase-h, very specific to rna attached to dna

disadvantgae of cdna

only has a small amount of information and representation

procedure to clone dna restriction fragments into a vector