L4.2 HEMA - Blood Cell Morphology & Hematopoiesis

Pronormoblast

Basophilic Normoblast

Orthochromatic Normoblast

Reticulocyte

Mature RBC

5 Terms under Maturation of Erythrocytes (Erythropoiesis)

(P, BN, ON, R, MRBC)

Pro

Basophilic

chromatic

Reticulocyte

5 TERMS UNDER MATURATION OF ERYTHROCYTES (ERYTHROPOIESIS):

.

↳ ____normoblast

.

↳ ___________ Normoblast

.

↳ Ortho__________ Normoblast

.

↳ ___________

.

↳ Mature RBC

Pronormoblast

MATURATION OF ERYTHROCYTES (ERYTHROPOIESIS):

.

↳ 14 - 20 μm

.

↳ Cytoplasm = Deeply basophilic. Relatively small amount, appearing as a band around the nucleus. It may show a lighter staining area around the nucleus (perinuclear halo); non-granular.

.

↳ Nucleus = Relatively large. N/C ratio is about 8:1. Round or slight oval. Reddish. Fine chromatin pattern. Usually 1-2 nucleoli that are larger than those found in the myeloblast and may stain with a slight bluish tint.

20

basophilic

band

lighter

8:1

1

bluish

PRONORMOBLAST (RUBRIBLAST OR PROERYTHROBLAST):

.

↳ 14 - 2_ μm

.

↳ Cytoplasm = Deeply ____________. Relatively small amount, appearing as a ______ around the nucleus. It may show a ____________ staining area around the nucleus (perinuclear halo); non-granular.

.

↳ Nucleus = Relatively large. N/C ratio is about ____. Round or slight oval. Reddish. Fine chromatin pattern. Usually __- 2 nucleoli that are larger than those found in the myeloblast and may stain with a slight _________ tint.

Rubriblast or Proerythroblast

↳ Pronormoblast is aka... ________blast or Pro_____________

Basophilic Normoblast

MATURATION OF ERYTHROCYTES (ERYTHROPOIESIS):

.

↳ 12 - 17 μm

.

↳ Cytoplasm = Intensely basophilic. The amount of cytoplasm is relatively increased over the previous stage. Non-granular.

.

↳ Nucleus = Relatively large. N/C ratio is about 6:1. Round or slightly oval, centrally located. The chromatin pattern is slightly coarser than in the previous stage. Nucleoli are usually not visible if present.

12

Intensely

increased

6:1

centrally

coarser

BASOPHILIC NORMOBLAST (PRORUBRICYTE / BASOPHILIC ERYTHROBLAST):

.

↳ 1_ - 17 μm

.

↳ Cytoplasm = ______________ basophilic. The amount of cytoplasm is relatively _______________ over the previous stage. Non-granular.

.

↳ Nucleus = Relatively large. N/C ratio is about ____. Round or slightly oval, ___________ located. The chromatin pattern is slightly __________ than in the previous stage. Nucleoli are usually not visible if present.

Prorubricyte / Basophilic Erythroblast

↳ Basophilic Normoblast is aka... Pro__________ / Basophilic _____________

Orthochromatic Normoblast

MATURATION OF ERYTHROCYTES (ERYTHROPOIESIS):

.

↳ 7 - 12 μm

.

↳ Cytoplasm = Pinker than the previous stage because of increased hemoglobin. It shows increased amount compared to previous stage. Non-granular.

.

↳ Nucleus = N/C ratio is about 1:2; small pyknotic nucleus (a homogenous blue-black mass with no structure). This is the primary difference between the rubricyte and metarubricyte. Nucleus may be bizarre in form and partially extruded from the cell.

12

Pinker

increased

1:2

pyknotic

black

bizarre

ORTHOCHROMATIC NORMOBLAST (METARUBRICYTE / ORTHOCHROMATIC ERYTHROBLAST):

.

↳ 7 - __2 μm

.

↳ Cytoplasm = _________ than the previous stage because of increased hemoglobin. It shows _____________ amount compared to previous stage. Non-granular.

.

↳ Nucleus = N/C ratio is about ___; small ___________ nucleus (a homogenous blue-_______ mass with no structure). This is the primary difference between the rubricyte and metarubricyte. Nucleus may be __________ in form and partially extruded from the cell.

Metarubricyte / Orthochromatic Erythroblast

↳ Orthochromatic Normoblast is aka Meta_____________ / Orthochromatic ______________.

Reticulocyte

MATURATION OF ERYTHROCYTES (ERYTHROPOIESIS):

.

↳ 7 - 10 μm

.

↳ Cytoplasm = Pink to a slightly pinkish gray. Contains a fine basophilic reticulum of RNA, which only stains with supraviral stains. There should be 2 or more reticular dots.

.

↳ Nucleus = Non Present.

10

pinkish

reticulum

supraviral

reticular

RETICULOCYTE (POLYCHROMATOPHILIC ERYTHROCYTE):

.

↳ 7 - __ μm

.

↳ Cytoplasm = Pink to a slightly ___________ gray. Contains a fine basophilic ____________ of RNA, which only stains with ______________ stains. There should be 2 or more ____________ dots.

.

↳ Nucleus = Non Present.

Polychromatophilic Erythrocyte

↳ Reticulocyte is aka...

Mature RBC (Erythrocyte)

MATURATION OF ERYTHROCYTES (ERYTHROPOIESIS):

.

↳ 6 - 8 μm

.

↳ Cytoplasm = Pink to peach in color. The mature RBC is a non-nucleated, round, biconcave disc.

.

↳ Nucleus = Non Present.

8

peach

biconcave

MATURE RBC (ERYTHROCYTE):

.

↳ 6 - _ μm

.

↳ Cytoplasm = Pink to __________ in color. The mature RBC is a non-nucleated, round, ____________ disc.

.

↳ Nucleus = Non Present.

Myeloblast

Promyeloblast

Myelocyte

Metamyelocyte

Band Cell

Segmented Neutrophil

Eosinophil

Basophil

8 Terms under Maturation of Granulocytes (Granulopoiesis)

(M, P, M, M, BC, SN, E, B)

Myeloblast

cyte

Meta

Band

Neutrophil

Basophil

8 TERMS UNDER MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ __________

.

↳ Promyeloblast

.

↳ Myelo_____

.

↳ _____myelocyte

.

↳ ______ Cell

.

↳ Segmented _____________

.

↳ Eosinophil

.

↳ ________

Myeloblast

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 12 - 20 μm

.

↳ Cytoplasm = Small amount in relation to the rest of the cell. Usually moderate blue in color. Texture is smooth and usually non-granular.

.

↳ Nucleus = Round or slight oval. Occupies about four-fifths of the cells. N/C ratio of 4:1. Extremely fine chromatin pattern. Reddish purple in color. It contains 2 or 5 nucleoli.

20

Small

blue

four

4:1

fine

Reddish

5

MYELOBLAST:

.

↳ 12 - _0 μm

.

↳ Cytoplasm = _______ amount in relation to the rest of the cell. Usually moderate ______ in color. Texture is smooth and usually non-granular.

.

↳ Nucleus = Round or slight oval. Occupies about ______-fifths of the cells. N/C ratio of ____. Extremely _____ chromatin pattern. __________ purple in color. It contains 2 or __ nucleoli.

Promyeloblast

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 15 - 21 μm

.

↳ Cytoplasm = Pale blue to basophilic. Contains a few to many, large blue to reddish purple staining non-specific (primary) granules.

.

↳ Nucleus = Occupies half or more of the cell. N/C ratio of 3:1 to 2:1. Oval or round. Chromatin pattern may become a little coarser, although it will still be relatively fine. 2 or 3 nucleoli are present.

21

Pale

reddish

half

3:1

coarser

3

PROMYELOBLAST:

.

↳ 15 - 2_ μm

.

↳ Cytoplasm = _______ blue to basophilic. Contains a few to many, large blue to ___________ purple staining non-specific (primary) granules.

.

↳ Nucleus = Occupies ______ or more of the cell. N/C ratio of ____ to 2:1. Oval or round. Chromatin pattern may become a little _________, although it will still be relatively fine. 2 or __ nucleoli are present.

Myelocyte

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 12 - 18 μm

.

↳ Cytoplasm = Moderate amount. May contain a few patches of blue. Few to moderate number of non specific granules. Small, specific granules begin to appear in this stage.

.

↳ Nucleus = Chromatin patterns become coarser and more condensed. Generally, it shows no nucleoli. Nucleus may be centrally located or may be eccentric. N/C ratio is 1:1.

18

Moderate

blue

specific

condensed

centrally

1:1

MYELOCYTE:

.

↳ 12 - _8 μm

.

↳ Cytoplasm = ____________ amount. May contain a few patches of ______. Few to moderate number of non __________ granules. Small, specific granules begin to appear in this stage.

.

↳ Nucleus = Chromatin patterns become coarser and more ______________. Generally, it shows no nucleoli. Nucleus may be _____________ located or may be eccentric. N/C ratio is ___.

Neutrophilic Myelocyte

Eosinophilic Myelocyte

Basophilic Myelocyte

3 Types of Myelocyte

(NM, EM, BM)

Neutrophilic Myelocyte

TYPE OF MYELOCYTE:

.

↳ The pink specific granules may be seen as pinkish or lighter stain areas in the cytoplasm, usually appearing near the nuclei first.

pink

lighter

first

NEUTROPHILIC MYELOCYTE:

.

↳ The _______ specific granules may be seen as pinkish or __________ stain areas in the cytoplasm, usually appearing near the nuclei _______.

Eosinophilic Myelocyte

TYPE OF MYELOCYTE:

.

↳ The specific granules first appear as dirty orange to blue. These granules are larger than the specific and non specific granules of the neutrophil.

orange

specific

EOSINOPHILIC MYELOCYTE:

.

↳ The specific granules first appear as dirty __________ to blue. These granules are larger than the specific and non ___________ granules of the neutrophil.

Basophilic Myelocyte

TYPE OF MYELOCYTE:

.

↳ The specific granules are few, large and are colored dark blue-purple.

large

blue

BASOPHILIC MYELOCYTE:

.

↳ The specific granules are few, ________ and are colored dark ______-purple.

Metamyelocyte (Juvenile)

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 10 - 15 μm

.

↳ Cytoplasm = Moderate to abundant amount giving a decreased N/C ratio. Few non-specific granules are present.

.

↳ Nucleus = Indented or kidney-shaped. The chromatin pattern is coarse and clumped and stained dark purple.

15

Moderate

decreased

specific

kidney

dark

METAMYELOCYTE (JUVENILE):

.

↳ 10 - _5 μm

.

↳ Cytoplasm = ___________ to abundant amount giving a _____________ N/C ratio. Few non-__________ granules are present.

.

↳ Nucleus = Indented or _________-shaped. The chromatin pattern is coarse and clumped and stained _____ purple.

Neutrophilic Metamyelocyte

Eosinophilic Metamyelocyte

Basophilic Metamyelocyte

3 Types of Metamyelocyte

(NM, EM, BM)

Neutrophilic Metamyelocyte

TYPE OF METAMYELOCYTE:

.

↳ The granules are lilac and are more numerous.

lilac

numerous

NEUTROPHILIC METAMYELOCYTE:

.

↳ The granules are _______ and are more _____________.

Eosinophilic Metamyelocyte

TYPE OF METAMYELOCYTE:

.

↳ The granules are brighter orange-red and more numerous.

orange

EOSINOPHILIC METAMYELOCYTE:

.

↳ The granules are brighter __________-red and more numerous.

Basophilic Metamyelocyte

TYPE OF METAMYELOCYTE:

.

↳ The dark-purple to black granules are more numerous.

black

BASOPHILIC METAMYELOCYTE:

.

↳ The dark-purple to _______ granules are more numerous.

Band Cell

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 10 - 15 μm

.

↳ Cytoplasm = Same as the metamyelocyte.

.

↳ Nucleus = Elongated or band shaped. Deeply indented than the metamyelocyte stage. Chromatin is coarse and clumped.

10

metamyelocyte

Elongated

indented

coarse

BAND CELL:

.

↳ _0 - 15 μm

.

↳ Cytoplasm = Same as the ___________________.

.

↳ Nucleus = _____________ or band shaped. Deeply __________ than the metamyelocyte stage. Chromatin is __________ and clumped.

Stab / Staff Cell

↳ Band Cell is aka... _____ / ______ Cell

Segmented Neutrophil

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 9 - 15 μm

.

↳ Cytoplasm = Full complement of lilac to rose-violet specific granules. Abundant amount not covering its nucleus.

.

↳ Nucleus = Usually has 2 - 5 lobes.

9

violet

nucleus

5

SEGMENTED NEUTROPHIL:

.

↳ _ - 15 μm

.

↳ Cytoplasm = Full complement of lilac to rose-_________ specific granules. Abundant amount not covering its __________.

.

↳ Nucleus = Usually has 2 - __ lobes.

Polymorphonuclear Cell

↳ Segmented Neutrophil is aka Poly_________________ Cell.

Eosinophil

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 10 - 15 μm

.

↳ Cytoplasm = Contains the full complement of large, reddish-orange granules not covering its nucleus.

.

↳ Nucleus = May possess 2 - 3 lobes, usually bi-lobed. Chromatin pattern is coarse and clumped.

15

reddish

2

bi-lobed

EOSINOPHIL:

.

↳ 10 - _5 μm

.

↳ Cytoplasm = Contains the full complement of large, __________-orange granules not covering its nucleus.

.

↳ Nucleus = May possess __ - 3 lobes, usually __________. Chromatin pattern is coarse and clumped.

Basophil

MATURATION OF GRANULOCYTES (GRANULOPOIESIS):

.

↳ 9 - 14 μm

.

↳ Cytoplasm = Stains slightly pink to colorless. Contains specific dark purple to blue-black granules that cover the nucleus.

.

↳ Nucleus = Usually possesses a stab-shaped nucleus.

14

colorless

specific

blue

stab

BASOPHIL:

.

↳ 9 - 1_ μm

.

↳ Cytoplasm = Stains slightly pink to _____________. Contains __________ dark purple to ______-black granules that cover the nucleus.

.

↳ Nucleus = Usually possesses a ______-shaped nucleus.

Lymphoblast

Pro-Lymphocyte

Mature Lymphocyte

Small Lymphocyte

Medium Lymphocyte

Large Lymphocyte

6 Terms under Maturation of Lymphocytes (Lymphopoiesis)

(L, PL, ML, SL, ML, LL)

blast

Pro

Mature

Medium

6 TERMS UNDER MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ Lympho_____

.

↳ ____-Lymphocyte

.

↳ _________ Lymphocyte

.

↳ Small Lymphocyte

.

↳ _________ Lymphocyte

.

↳ Large Lymphocyte

Lymphoblast

MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ 10 - 18 μm

.

↳ Cytoplasm = No granules are present. Appears smooth. Moderate to dark blue.

.

↳ Nucleus = Chromatin pattern is somewhat coarse. Round or oval. Contains 1 or 2 distinct nucleoli. N/C ratio is 4:1.

10

smooth

blue

coarse

2

4:1

LYMPHOBLAST:

.

↳ 1_ - 18 μm

.

↳ Cytoplasm = No granules are present. Appears __________. Moderate to dark _____.

.

↳ Nucleus = Chromatin pattern is somewhat ________. Round or oval. Contains 1 or __ distinct nucleoli. N/C ratio is ____.

Pro-Lymphocyte

MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ Same as lymphoblast or smaller.

.

↳ Cytoplasm = Moderate blue to dark blue. Non-granular. May contain occasional azurophilic granules.

.

↳ Nucleus = Round, oval or slightly indented. Chromatin pattern is more clumped than lymphoblast. May contain 1 - 2 nucleoli.

smaller

dark

azurophilic

indented

clumped

2

PRO-LYMPHOCYTE:

.

↳ Same as lymphoblast or _________.

.

↳ Cytoplasm = Moderate blue to ______ blue. Non-granular. May contain occasional _______________ granules.

.

↳ Nucleus = Round, oval or slightly ___________. Chromatin pattern is more ___________ than lymphoblast. May contain 1 - __ nucleoli.

Mature Lymphocyte

MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ 8 - 16 μm

.

↳ Cytoplasm = Generally, the larger the lymphocyte, the more abundant the cytoplasm.

.

↳ Nucleus = None.

16

abundant

MATURE LYMPHOCYTE:

.

↳ Size variation of 8 - 1_ μm.

.

↳ Cytoplasm = Generally, the larger the lymphocyte, the more ____________ the cytoplasm.

.

↳ Nucleus = None.

Small Lymphocyte

MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ 6 - 10 μm

.

↳ Cytoplasm = Forms a thin rim around the nucleus. Moderate blue to dark blue.

.

↳ Nucleus = Chromatin pattern is dense and clumped. Round or oval and may be slightly indented. No nucleoli visible.

6

thin

dark

dense

nucleoli

SMALL LYMPHOCYTE:

.

↳ __ - 10 μm

.

↳ Cytoplasm = Forms a _____ rim around the nucleus. Moderate blue to ______ blue.

.

↳ Nucleus = Chromatin pattern is _______ and clumped. Round or oval and may be slightly indented. No ___________ visible.

Medium Lymphocyte

MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ 10 - 12 μm

.

↳ Cytoplasm = More abundant than that of small lymphocytes. Pale to moderate blue. May or may not contain a few non-azurophilic granules.

.

↳ Nucleus = Round, oval or slightly indented. Chromatin pattern is clumped but not as dense as small lymphocytes. No nucleolus is visible.

12

abundant

moderate

azurophilic

slightly

dense

nucleolus

MEDIUM LYMPHOCYTE:

.

↳ 10 - 1_ μm

.

↳ Cytoplasm = More _____________ than that of small lymphocytes. Pale to ____________ blue. May or may not contain a few non-_____________ granules.

.

↳ Nucleus = Round, oval or _________ indented. Chromatin pattern is clumped but not as _______ as small lymphocytes. No _____________ is visible.

Large Lymphocyte

MATURATION OF LYMPHOCYTES (LYMPHOPOIESIS):

.

↳ 12 - 16 μm

.

↳ Cytoplasm = Abundant, clear, very pale blue. May or may not contain few non specific azurophilic granules.

.

↳ Nucleus = Round, oval or slightly indebted. Chromatin pattern is coarse. No nucleolus visible.

16

pale

specific

coarse

LARGE LYMPHOCYTE:

.

↳ 12 - 1_ μm

.

↳ Cytoplasm = Abundant, clear, very _______ blue. May or may not contain few non __________ azurophilic granules.

.

↳ Nucleus = Round, oval or slightly indebted. Chromatin pattern is ________. No nucleolus visible.

Plasmablast

Pro-Plasmacyte

Plasmacyte (Plasma Cell)

3 Terms under Maturation of Plasma Cells (Plasmacytic Series)

(P, PP, P/PC)

Plasmablast

MATURATION OF PLASMA CELLS (PLASMACYTIC SERIES):

.

↳ 18 - 15 μm

.

↳ Cytoplasm = Basophilic cytoplasm. Abundant. Non-granular.

.

↳ Nucleus = Chromatin pattern is more clumped than lymphocyte. Eccentric or located off center. Perinuclear halo may be present. Round or oval. Multiple nucleoli may or may not be visible.

18

Basophilic

granular

clumped

center

halo

Multiple

PLASMABLAST:

.

↳ 1_ - 15 μm

.

↳ Cytoplasm = ____________ cytoplasm. Abundant. Non-__________.

.

↳ Nucleus = Chromatin pattern is more __________ than lymphocyte. Eccentric or located off _______. Perinuclear _____ may be present. Round or oval. ___________ nucleoli may or may not be visible.

Pro-Plasmacyte

MATURATION OF PLASMA CELLS (PLASMACYTIC SERIES):

.

↳ 15 - 25 μm

.

↳ Cytoplasm = Intensely basophilic. Non-granular. Bluer than plasmablast. A lighter staining area in the cytoplasm, next to the nucleus if often visible (hof or perinuclear halo).

.

↳ Nucleus = Round, oval or slightly. Eccentrically placed. Chromatin is coarse and densely stained. Occasional nucleoli may be seen.

25

Intensely

Bluer

lighter

perinuclear

Occasional

PRO-PLASMACYTE:

.

↳ 15 - _5 μm

.

↳ Cytoplasm = _______________ basophilic. Non-granular. _______ than plasmablast. A __________ staining area in the cytoplasm, next to the nucleus if often visible (hof or _______________ halo).

.

↳ Nucleus = Round, oval or slightly indented. Eccentrically placed. Chromatin is coarse and densly stained. _____________ nucleoli may be seen.

Plasmacyte (Plasma Cell)

MATURATION OF PLASMA CELLS (PLASMACYTIC SERIES):

.

↳ 8 - 20 μm

.

↳ Cytoplasm = Deeply basophilic. Large and with a well-defined hof near the nucleus which is actually the golgi apparatus. Usually non granular. May contain immunoglobulins.

.

↳ Nucleus = Round or oval. Eccentrically placed. Chromatin is heavily clumped, which is sharply defined from parachromatin, often arranged in radial or spokes wheel pattern.

20

hof

apparatus

globulins

para

wheel

PLASMACYTE (PLASMA CELL):

.

↳ 8 - _0 μm

.

↳ Cytoplasm = Deeply basophilic. Large and with a well-defined ____ near the nucleus which is actually the golgi _____________. Usually non granular. May contain immuno_____________.

.

↳ Nucleus = Round or oval. Eccentrically placed. Chromatin is heavily clumped, which is sharply defined from _____chromatin, often arranged in radial or spokes _________ pattern.

Monoblast

Promonocyte

Monocyte

3 Terms under Maturation of Monocytes (Monopoiesis)

(M, P, M)

Monoblast

MATURATION OF MONOCYTES (MONOPOIESIS):

.

↳ 14 - 20 μm

.

↳ Cytoplasm = Moderately basophilic to blue-gray. Non-granular.

.

↳ Nucleus = Ovoid or round. Light blue-purple. Chromatin is fine, lacey. 1 or 2 nucleoli are present. N/C ratio is 4:1 to 3:1.

20

basophilic

Ovoid

purple

lacey

3:1

MONOBLAST:

.

↳ 14 - _0 μm

.

↳ Cytoplasm = Moderately ______________ to blue-gray. Non-granular.

.

↳ Nucleus = ______ or round. Light blue-_________. Chromatin is fine, ______. 1 or 2 nucleoli are present. N/C ratio is 4:1 to ____.

Promonocyte

MATURATION OF MONOCYTES (MONOPOIESIS):

.

↳ 14 - 18 μm

.

↳ Cytoplasm = Blue-gray. Contain fine dustlike azurophilic granules. Shows ground glass appearance.

.

↳ Nucleus = None.

18

Blue

dust

glass

PROMONOCYTE:

.

↳ 14 - _8 μm

.

↳ Cytoplasm = ______-gray. Contain fine _____like azurophilic granules. Shows ground _______ appearance.

.

↳ Nucleus = None.

Monocyte

MATURATION OF MONOCYTES (MONOPOIESIS):

.

↳ 14 - 18 μm

.

↳ Cytoplasm = Blue-gray. Presence of pseudopods. Fine azurophilic granule, giving a ground glass appearance. Vacuole may be present.

.

↳ Nucleus = Round, kidney-shaped. Slight lobulation. Shows brain-like convolutions. No nucleolus is visible. Chromatin is fine and delicate and lacey arranged in skein-like strands.

18

pseudopods

Vacuole

kidney

lobulation

brain

skein

MONOCYTE:

.

↳ 14 - ___ μm

.

↳ Cytoplasm = Blue-gray. Presence of _____________. Fine azurophilic granule, giving a ground glass appearance. ___________ may be present.

.

↳ Nucleus = Round, _________-shaped. Slight ____________. Shows _______-like convolutions. No nucleolus is visible. Chromatin is fine and delicate and lacey arranged in ________-like strands.

Megakaryoblast (Stage I)

Promegakaryocyte (Stage II)

Granular Megakaryocyte (Stage III)

Platelet (Thrombocyte)

4 Terms under Maturation of Megakaryocytes (Megakaryopoiesis)

M, P, GM, P

Pro

Granular

4 TERMS UNDER MATURATION OF MEGAKARYOCYTES (MEGAKARYOPOIESIS):

.

↳ Megakaryoblast (Stage I)

.

↳ ____megakaryocyte (Stage II)

.

↳ __________ Megakaryocyte (Stage III)

.

↳ Platelet (Thrombocyte)

Megakaryoblast (Stage I)

MATURATION OF MEGAKARYOCYTES (MEGAKARYOPOIESIS):

.

↳ 20 - 50 μm

.

↳ Cytoplasm = Shades of blue. Have small blunt pseudopods. Narrow bond around the nucleus. Non-granular. As the cell matures, the amount of cytoplasm increases.

.

↳ Nucleus = Round or oval or kidney-shaped. Chromatin pattern is fine. Multiple nucleoli. Stained blue. N/C ratio is 10:1.

50

blue

blunt

cytoplasm

kidney

10:1

MEGAKARYOBLAST (STAGE I):

.

↳ 20 - _0 μm

.

↳ Cytoplasm = Shades of ______. Have small _______ pseudopods. Narrow bond around the nucleus. Non-granular. As the cell matures, the amount of ______________ increases.

.

↳ Nucleus = Round or oval or __________-shaped. Chromatin pattern is fine. Multiple nucleoli. Stained blue. N/C ratio is _____.

Promegakaryocyte (Stage II)

MATURATION OF MEGAKARYOCYTES (MEGAKARYOPOIESIS):

.

↳ 20 - 60 μm

.

↳ Cytoplasm = Less basophilic. Granules begin to form in the golgi region.

.

↳ Nucleus = Chromatin becomes coarse. Multiple nucleoli. Irregular in shape. Slight lobulation. N/C ratio is 4:1 to 7:1.

60

Less

golgi

Irregular

7:1

PROMEGAKARYOCYTE (STAGE II):

.

↳ 20 - _0 μm

.

↳ Cytoplasm = _____ basophilic. Granules begin to form in the ________ region.

.

↳ Nucleus = Chromatin becomes coarse. Multiple nucleoli. ____________ in shape. Slight lobulation. N/C ratio is 4:1 to ____.

Granular Megakaryocyte (Stage III)

MATURATION OF MEGAKARYOCYTES (MEGAKARYOPOIESIS):

.

↳ 30 - 90 μm

.

↳ Cytoplasm = Pinkish in color. Very fine. Abundant. Reddish blue granules. Irregular border.

.

↳ Nucleus = Multiple nuclei. Multiple lobulation. Chromatin is coarser. No nucleolus. N/C ratio is 1:1.

90

Pinkish

blue

border

lobulation

1:1

GRANULAR MEGAKARYOCYTE (STAGE III):

.

↳ 30 - _0 μm

.

↳ Cytoplasm = __________ in color. Very fine. Abundant. Reddish ______ granules. Irregular _________.

.

↳ Nucleus = Multiple nuclei. Multiple ___________. Chromatin is coarser. No nucleolus. N/C ratio is ___.

Platelet (Thrombocyte)

MATURATION OF MEGAKARYOCYTES (MEGAKARYOPOIESIS):

.

↳ 2 - 5 μm

.

↳ Cytoplasm = Light blue to purple. Very granular that consists of: Granulomere/Chromomere which is located centrally and; Hyalomere which surrounds the chromomere.

.

↳ Nucleus = None.

5

purple

Granulomere

centrally

Hyalomere

PLATELET (THROMBOCYTE):

.

↳ 2 - __ μm

.

↳ Cytoplasm = Light blue to ___________. Very granular that consists of: ________________/Chromomere which is located __________ and; _____________ which surrounds the chromomere.

.

↳ Nucleus = None.