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describe role of RNA polymerase in transcription of a gene (2024 j1 tmjc promos)
Initiation
1. Protein A recognizes and binds to the promoter to initiate transcription.
2. Protein A unwinds and unzips the double-stranded DNA molecule.
Elongation
3. Protein A reads the DNA template strand from the 3' to 5' direction...
4....and adds (ribo)nucleotides in the 5' to 3' direction by complementary base pairing.
5. Protein A catalyses the formation of phosphodiester bonds between adjacent (ribo)nucleotides
Termination
6. Protein A transcribes a terminator sequence in the DNA template strand.
7. Protein A detaches from the DNA and releases the mRNA strand (at least one from each part)
d
a
a