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which factor deficiency will not be detected by the APTT
VII
VIII
V
II
a
what is the anticoagulant of choice for cell counting and morphological assessment
EDTA K3
starch
a pt recieved 2 units of O Rh pos un xm blood. a blood specimen for a xm was collected and two more units of blood were ordered. the following results were obtained as part of the xm request:
anti-A= 3+ mf
anti-B= 0
anti-A,B= 3+ mf
A1 cells= 0
B cells=4+
O cells=0
which of the following is the most likely explanation for the results?
Pt has multiple myeloma
There is more than one cell population
The patient is a rare subgroup of A
b
in an emergency situation where the patient’s ABO and Rh group have been determined and they require an immediate rbc transfusion. theres no time for a xm. what ABO/Rh should be used
blood of same ABO and Rh
what should be done when a hemolyzed sample is recieved for SPE
repeat test using new sample from same patient
dilute hemolyzed sample and procees with testing
reject specimen and request a new sample from the pt
perform test as usual, noting hemolysis on the report
c
which performance study in the method evaluation process assesses random variation
Linearity study
calibration study
method comparison study
precision study
d
a texhnologist is performing a manual wbc count and notices the sample is clotted. what action should be taken?
centrifuge the sample at a higher speed to remove clots.
perform the count using a different diluent to dissolve clots.
proceed with the count using the clotted sample
reject the sample and request recollection
d
why is specific gravity (SG) testing often included with urine pregnancy testing?
to detect the presence of proteins that might interfere with the test
to confirm the presence of hCG in the urine sample
to measure the hydration level of the patient
to ensure the urine sample is concentrated enough for accurate test results
d
what should the technologist do when a stat specimen is received for an APTT in an EDTA tube?
perform APTT as requested
request a repeat specimen
dilute the specimen 50% with isotonic saline
transfer specimen to a sodium citrate tube before testing
b
when performing microscopic urinalysis, which of the following is the best method to differentiate yeast from rbcs
use higher magnification to identify cell size differences
perform urine dipstick test for blood
examine for a distinct biconcave shape to id yeast
observe for budding forms to id yeast
d
what serum protein electrophoresis (SPE) pattern is commonly seen in cirrhosis
beta gamma bridging
what serum protein electrophoresis (SPE) pattern is commonly seen in hypogammaglobulinemia/agammaglobulinemia
decreased gamma globulin and sharp albumin peak
what serum protein electrophoresis (SPE) pattern is commonly seen in acute phase rxn/acute inflammatory response
elevated alpha 1 and alpha 2 globulins with a normal beta region
what serum protein electrophoresis (SPE) pattern is commonly seen in MGUS, monoclonal gammopathy, multiple myeloma
increased albumin with an M spike in the gamma rgn
group o patient requires a plasma tranfusion, but NO group O frozen plasma is available. Which Best describes acceptable alternative group(s) of plasma products
group A, B, AB are all acceptable alternatives
a decreased bicarb level in the blood without a change in PCO2 will result in what acid/base imbalance
metabolic acidosis
a wright’s stained peripheral blood smear shown. the bone marrow aspirate of this patient demonstrated an abundance of cells with this morphology. which of the following conditions is most likely associated with this sample?
multiple myeloma
what cell
sezary cell
which of the following actions should be taken in the event of a CSF spill
cover the spill with paper towels and soak with hypochlorite
clean up the specimen using a chemical spill kit
absorb the spill with paper towels which are then autoclaved
recover the precious specimen with a pipette for testing
a
which analyte requires a fasting specimen
potassium
creatinine
triglycerides
urea
triglycerides
how is a glucose value of 30.0 mmol/L interpreted
critical
what can reconstituting a lyophilized control vial with too much diluent result in
QC results lower than the expected range
No effect on QC results
accurate QC results w/in the expected range
QC results higher than the expected range
a
what is the plt count when an avg of 300 plta were counted on a hemocytometer where 20 uL of blood was added to 1.98 mL of ammonium oxalate
300 × 10^9/L
what effect does increasing the angle of the spreader slide have on the blood smear produced
thicker smear
what range must the control values fall between if a ctrl has a mean of 4.0 with a SD of 0.5 and the lab is using a 95.5% confidence interval
3.0 to 5.0
tube w a green stopper normally contains
heparin
if a pt has a normal APTT and abnormal PT, which coag factor is most likely deficient
V
VII
I
II
b
what is the primary purpose of using an internal standard in gas chromatography
to improve the resolution of chomatographic peaks
to calibrate the detector for consistent sensitivity
to correct for variations in sample volume or injection amount
to reduce the time required for analysis
c
what are 8-10 random, consecutive values all lying abv or all below the mean on a levy jennings chart called
shift
a plasma sample was analyzed using ion selective electrodes (ISE) and the following electrolyte results were obtained: [Na= 140 mmol/L; K+= 14.0 mmol/L; Cl-= 112 mm/L; HCO3-= 18 mmol/L]. What action should be taken next?
Check the range to determine if the sample should be diluted and rerun for an accurate potassium result
The sample was slightly hemolyzed so request a new sample.
Suspect improper anticoagulant; check collection tube and redraw
Report the potassium result to the physician immediately
b
what is demonstrated by polarized microscopy
Luminescence
Phosphorescence
birefringence
fluorescence
birefringence
which of these inclusions CANT be seen on a romanowsky stained blood film
basophilic stippling
howell jolly bodies
heinz bodies
cabot rings
c
which of the following urinalysis results are discrepant
Moderate blood but no RBC in microscope
negative blood but pos protein
small blood but negative protein
negative blood but 10-15 rbcs /hpf
d
this test requires a specimen with a 9:1 ratio of blood to anticoagulant
glycohoglobin
ethanol
zinc
prothrombin time
d
given the following info what would be the calculated osmolality [Na= 135 mmol/L; Cl= 110 mmol/L; CO2 = 15 mmol/L; Glu= 5.0 mmol/L; Urea= 5.0 mmol/L; Ethanol= 35 mmol/L
280
which of the following conditions is cause for rejecting an analytical run?
one ctrl greater than 2SD
2 consecutive controls greater than 2SD units above and below the mean
3 consecutive controls greater than 1SD unit from the mean
4 ctrls increasing in value but less than 1SD unit from the mean
b
what is the area of the large square on one side of a hemocytometer
9 mm²
a 17 y o female requires two units of blood immediately and there is insufficient time to perform any testing. 2 yrs previous she tested group A Rh positive. Which of the following rbcs would be issued?
O neg
A pos
A neg
O pos
a
during analysis of a synovial fluid sample, a technologist notices the presence of crystals. what should the technologist do next?
reject the sample and request recollection
dilute the sample before testing to reduce crystal concentration
report the finding as normal
perform a polarized light microscopy to id the crystal type
d
which coag factor deficiency can be treated with vitamin K
XI
VIII
I
II
II
what cbc parameter will be increased when a lipemic sample is processed
MCHC
MCV
RDW
RBC
MCHC
what conditions should be suspected from the peripheral blood smear
iron deficiency anemia
in a cbc (complete blood count), which parameter reflects the percentage of total blood volume occupied by rbcs
HCT
a technologist is performing a coagulation test and notices that the specific volume is insufficient. what is the appropriate action?
request recollection of the specimen
which of the following is an important consideration when performing dipstick testing on urine?
the dipstick should be immersed in the urine sample for at least 5 mins
the urine sample should be collected at any time of the day
the dipstick should be stored directly in the urine sample for accurate results
the urine sample should be well mixed before testing
d
what is the significance of the presence of paraproteins in SPE
they indicate a normal variation in protein lvls
they suggest the presence of a monoclonal gammopathy, such as multiple myeloma
they are associated with acute inflammatory conditions
they indicate liver dysfunction or damage
b
plt count of 25 × 10^9/L is obtained on a sample. which of the following should be the technologist’s first action
telephone the result to the ward
repeat the count on a new specimen
repeat the count using a second instrument
verify the count microscopically
d
what is agreement between the best estimate of a quantity and its true value?
reliability
precision
accuracy
standard deviation
c
if you need to make a call for info, what should you do before you call
brush ur teef
prepare ur questions
make coffee
chew gum
b
a serum protein electrophoresis is performed. why does the technologist suspect that the sample used was plasma, not serum?
bc of a characteristic peak btw the beta and gamma regions
bc of a characteristic peak between the albumin and alpha 1 regions
bc of a characteristic peak between the alpha2 and beta region
bc of characteristic peak btw the alpha1 and alpha2 region
a
a listless 12 month old boy with a fever of 103 deg C was taken to the emergency department. he had been diagnosed with an ear infection 3 days earlier. A spinal tap was performed, but only one tube of CSF was obtained from the lumbar puncture. Which department should be the first to recieve the single tube of CSF
micro
the presence of schistocytes on an impedance analyser will cause a false increase in
WBC count
plt count
retic count
mean cell volume
b
what additional test is required when a retic count is ordered
red blood cell count
the cells seen below in the wright’s stained peripheral blood smear are diagnostic of which condition
sickle cell disease
a pt gives an APTT of 75 sec. An APTT mixing study is performed and the result is still 75 secs. whats the reason for these results?
Multiple factor deficiencies
factor I deficiency
factor IX deficiency
A circulating anticoagulant
d
blood products must be transfused a maximum of how many hours after issue
24
4
8
6
4