Step by step glycolysis

What is the enzyme responsible for catalyzing the first reaction of glycolysis, where glucose is phosphorylated to glucose-6-phosphate?

Hexokinase

What type of reaction occurs during the conversion of glucose to glucose-6-phosphate?

Phosphorylation

Which enzyme catalyzes the conversion of glucose-6-phosphate into fructose-6-phosphate?

Phosphoglucose isomerase

The reaction catalyzed by phosphoglucose isomerase is best described as:

An isomerization reaction

Which enzyme catalyzes the second ATP-consuming step in glycolysis, converting fructose-6-phosphate into fructose-1,6-bisphosphate?

Phosphofructokinase

The phosphofructokinase reaction is:

Exergonic and irreversible

Which enzyme catalyzes the cleavage of fructose-1,6-bisphosphate into two three-carbon molecules, glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP)?

Aldolase

The enzyme triose phosphate isomerase converts:

DHAP to G3P

Which enzyme catalyzes the oxidation and phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate?

Glyceraldehyde-3-phosphate dehydrogenase

Which molecule is reduced during the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase?

NAD+

Which enzyme catalyzes the first ATP-generating step of glycolysis by converting 1,3-bisphosphoglycerate into 3-phosphoglycerate?

Phosphoglycerate kinase

What type of phosphorylation occurs during the reaction catalyzed by phosphoglycerate kinase?

Substrate-level phosphorylation

Which enzyme catalyzes the rearrangement of 3-phosphoglycerate into 2-phosphoglycerate?

Phosphoglycerate mutase

The reaction catalyzed by phosphoglycerate mutase is best described as:

An isomerization reaction

Which enzyme catalyzes the dehydration of 2-phosphoglycerate into phosphoenolpyruvate (PEP)?

Enolase

What is the significance of the enolase reaction in glycolysis?

It forms a high-energy intermediate (PEP)

Which enzyme catalyzes the final ATP-generating step of glycolysis, converting phosphoenolpyruvate into pyruvate?

Pyruvate kinase

The pyruvate kinase reaction is:

Exergonic and irreversible