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Location of Glycolysis
Cytosol
Summary of Glycolysis
Splits glucose into 2 pyruvate
2 ATP used to attach 2 phosphates to glucose
Split into 2 glyceraldehyde-3-phosphate
NAD+ attaches another phosphate to each G3P (uses electron to become NADH)
Phosphate is given to ADP to become ATP
Net: 2 ATP + 2 NADH
Pyruvate oxidation happens where
mitochondrial matrix
if Oxygen is present, pyruvate enters mitochondria
Pyruvate oxidation summary
1 carbon of pyruvate (out of 3) is oxidized and released as CO2
Other 2 carbons are oxidized → the e- is sent to NAD+
Coenzyme A is attached by its sulfur group to the 2 carbons
Acetyl CoA is formed.
Net: 2 CO2 and 2 NADH
Citric Acid (Krebs) Cycle location
Mitochondrial Matrix
Citric Acid Cycle summary
2 carbon of acetyl CoA is attached to oxaloacetate (4 carbon molecule) to form Citrate (6 carbons)
2 carbons are released as CO2
Electrons are carried off into NADH (3) and FADH2 (1)
1 ATP is made as well
4 carbon oxaloacetate is regenerated
Repeat because 2 acetyl CoA’s
Net: 2 ATP, 6 NADH, 2 FADH2
Alcoholic ferm
release CO2 from pyruvate → become acetaldehyde (no oxygen for pyruvate oxidation)
Acetaldehyde is reduced by NADH to ethanol → regenerate NAD+ to do glycolysis again
Bacteria and yeast
Lactic Acid Ferm
pyruvate is reduced directly by NADH to form lactate
no CO2