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Spermatogenesis
Production and maintenance of sperm supply
Overall functions of the male
Produce and maintain sperm supply, detect females in estrus, inseminate females and fertilize the female gamete
Spermatic cord
Contains vas deferens, pampiniform plexus, and external cremaster muscle nerves
Vas Deferens
Transport sperm during ejaculation
Seminiferous Tubule
sperm producing cells- true germinal epithelium
Corpus Epididymis
Responsible for maturation of spermatozoa
Cauda Epididymis
Final maturation and storage, only sperm capable of fertilization
Tunica Albuginea
Connective tissue which holds testis together
Caput Epididymis
Function in maturation of spermatozoa, fluid absorbtion
Sertoli Cells
Support spermatogenesis
Leydig Cells
Testosterone synthesis
Function of Testes
1. Produce sperm
2. transport sperm out of testis
3. Produce testosterone
Testosterone function
1. Stimulate secondary sex characteristics, muscle development, and accessory glands
2. Maintains libido
3. Maintain function of male reproductive tract, penis, and muscles for ejaculation
Thermoregulation
Temperatures 3-5 degrees celsius less than the body temperature are required for spermatogenesis to occur
Scrotal properties that facilitate thermoregulation
1. Location of testis- outside away from the body air circulation
2. Low insulation- thin skin, low subcutaneous fat or hair
Pampiniform plexus
Counter-current heat exchange causes a cooling of arterial blood supply
Scrotum, testis, and spermatic cord
What all is involved in thermoregulation?
Cryptorchidism
lack of testis descent into scrotum and this thermoregulation is absent
Bilateral
Sterile, no spermatogenesis, testosterone is produced
Unilateral
Fertile, reduce sperm producing capacity
Functions of the epididymis
1. transport sperm
2. maturation of sperm in caput to corpus region
3. storage of sperm- cauda
Fate of unejaculated sperm
1. resorbed by excurrent duct system
2. sperm lost in urine
3. masturbation loss of sperm
Swine's volume of sperm
225-400
Horse's volume of sperm
60-100
Breeding soundness exam (BSE)
Fertility, body condition score, feet
Overall functions for the female reproductive tract
Control activity of organs (hormonal), transport sperm, produce oocyte, facilitate fertilization, provide environment for embryo and fetus, give birth to fetus, recycle to become pregnant again, provide nutrients to young
Vulva
passageway for urine, receptor for penis during copulation, passageway for fetus
Vestibule
Common duct for urine and reproduction, stimulates male for copulation, passage for fetus during parturition
Vagina
female organ of copulation at mating, birth canal at parturition, mucosal surface that changes during estrous cycle, copulatory organ, birth canal
Cervix
Thick wall extension of the uterus, structure is directly correlated to male penis, first sperm barrier in cow and ewe, transport sperm
Stallion ejaculation
Glans penis bells out and expands cervix, force of ejaculation sends semen directly into uterus
Boar penis
corkscrew shape, glans penis locks into the interdigitating pads of cervix
Functions of the cervix
barrier to sperm, reservoir for sperm, blocking bacterial invasion during pregnancy, birth canal
Uterus
incubator for fertilized ovum, aids travel of sperm, secretory organ, has two horns or branches
horns, body
Components of the uterus
Functions of the uterus
Transport sperm, muscle contractions, sperm motility, absorption and phagocytosis, partially prepares sperm for fertilization, provides environment for embryo, supports development of fetus, at birth expels fetus, recovers from pregnancy
Prostaglandin PGF2a
Hormone produced when not pregnant (by uterus)
Interferon tau
What signal is given off when pregnant?
Oviduct
Open-ended tube from area of ovary to uterus, passageway for sperm and ovum, site of fertilization, takes 3-5 days for ovum to travel down uterus
Oviductal functions
Transport sperm to site of fertilization, reduce sperm numbers to prevent polyspermy, remove oocyte from surface of ovary, transport oocyte to site of fertilization
Broad ligament
suspensory support for repro tract
Infundibulum
Catches ovum after ovulation, "catcher's mit"
Ovary
Produces female gamete, produces female sex hormones (estrogen & progesterone)
Cortex
Part of ovary that contains female germ cells, oocytes, follicles, corpora lutea (pl)
Medulla
Part of ovary that contains connective tissue, blood vessels, lymphatics, and nerves
Graffian
Mature follicle
Artretic
Deteriorated follicle
Corpus Hemorrhagicum (CH)
Ovarian structure, contains blood, lymph and granulosa cells, occurs before CL and CA
Corpus luteum (CL)
Ovarian structure, "yellow body" forms after ovulation, produces progesterone
Functions of progesterone
1. inhibits estrus and parturition
2. blocks myometrial contractions
3. stimulates endometrial secretion of nutrients
4. stimulates the production of a luteolytic agent to kill the CL if no embryo is present. Prostaglandin F2a
Corpus Albicans (CA)
Ovarian structure, white body, regressed corpus luteum, caused by prostaglandin
Luteinizing hormone (LH)
Secreted by: anterior pituitary
Acts upon: ovary
-Induces ovulation and development of the Corpus Luteum
Follicle stimulating hormone (FSH)
Secreted by: anterior pituitary
Acts upon: ovary
-Induces follicular development
Inhibin
Secreted by: Follicle
Acts upon: Anterior pituitary
-Causes reduction in FSH production
Estrogen
Secreted by: follicle
-Responsible for estrus, early stage development of the mammary gland
Progesterone
Secreted by: Corpus Luteum
"Hormone of pregnancy"
Oxytocin
Secreted by: Posterior pituitary, corpus luteum
Acts upon: smooth muscle
-Causes contractions of the small muscle fibers
Prostaglandin
Secreted by: Uterine lining
Acts upon: corpus luteum
-Causes the regression of the corpus luteum
Interferon tau
Produced by: fertilized ovum
Received by: uterus
-Pregnancy recognition signal
Why synchronize estrus?
Scheduled calving, more heats per season, problem cows are identified, early conception, increased heifer management, facilitates the use of A.I.
What does estrus synchronization help with?
Management, feed resources, facilities, uniform product, utilize labor
Ram effect
natural synchronization method
Flehmen response
Exterior response by male that tells when females are in estrus
Prostaglandins (in estrous synchronization)
cause leutolysis- shorten the current estrous cycle starting every animal at the same stage
Progestins (in estrous synchronization)
Keeps all animals at the same state until __________ are removed and they cycle together
Progesterone / Prostaglandin
_________________ is timing specific while _________________ is used as a management tool
Lutalyse
Common prostaglandin
PGF2a
What form of prostaglandin is used?
CIDR (controlled intervaginal drug release)
Progesterone insert commonly used to synchronize estrus in herds
-removed 7 days after insertion, cattle will show heat 24-48 hours after removal
MGA (melengasterol acetate)
Progesterone feed additive developed to suppress heat in feedlot heifers
Common signs of estrus
-Stands to be mounted by other animals
-Persistent trailing of other animals
-licking other animals
-Nervous and restless behavior
-red and swollen vulva
-mounting other animals
-clear mucus discharge
paint stick, gomer bull
Most common estrus detection aids
Artificial insemination
Placing semen into the reproductive tract of the female by artificial techniques rather than natural mating
Cow
What animal is artificially inseminated rectocervically?
-AM/PM rule
AM/PM rule
Universally accepted time for insemination of cows
Sow
What animal is artificially inseminated cervically?
Mare
What animal is artificially inseminated vaginocervially?
Sheep, goats, deer
What animal is artificially inseminated laparoscopically?
Goat
What animal is artificially inseminated with a speculum?
100%
What percent of poultry must be artificially inseminated?
Electro ejaculation
AI sperm collection method that stimulates nerve over bulbourethral gland
Extender solution
Components:
-Fructose- energy source
-Double distilled water
-Antibiotics
-Glycerol
-Egg yolk
Parturition
Action of giving birth, marks termination of pregnancy
Causes of dystocia
-Females are too young
-Abnormal presentations
-Abnormally small pelvis in female
-Fetus too large
AI Advantages
-Genetic improvement
-Disease control
-Improved record keeping
-Eliminates need for keeping bulls
AI Disadvantages
-Time required to detect estrus
-Percent of cows in estrus during the breeding season
-Trained personnel required
-Overuse of inferior sires
Embryo transfer
Movement of the pre-implantation embryos from the reproductive tract of the genetic mother (donor) to the reproductive tract of the surrogate mother (recipient)
Genetic gain (ET)
-Improvement in livestock through selective breeding
-Production of improved strains of livestock
Steps in ET process
-Super-ovulation
-Insemination
-Synchronization of donors and recipients (TIMING)
Follicular rescue
Induction of follicular development in excess of the normal number
FSH
What hormone is used to induce super-ovulation?
12 hours post estrus - 2 units
24 hours post estrus - 1 unit
Timing and units of semen for donor to be breed with
True
T/F: Recipient females must have synchronous estrous cycles with the the donor to achieve pregnancy.
7
How many days post estrus should embryos be collected in the bovine?
Growth
Increase in body weight until maturity is reached; increase in animal tissue: muscle, fat, and bone
True Growth
Only growth that involves an increase in structural tissues, excludes fat as storage tissue
Maturity
State of being fully grown or developed
Chronological age
animals age in terms of units of time
Physiological age
stages (landmarks) of development as the animal grows from conception to maturity
Hyperplasia
an increase in the number of cells