Medical Microbio MLS Unit One Review

Covers selective media, flagella, temperature, Nutrition, Some parts of immunity (goblet cell secretions, mucousal linings, Antibodies), Sterilization/Disinfection, Fume hood class, colony morph, pH indicators, some parts of flourescent microscopy, stains, lac operon

Bismuth Sulfite Agar (BSA)

Salmonella SPP

Buffered Charcoal Yeast Extract (BCYE)

Legionella

Cary-Blair Transport Medium

Enterics

Cetrimide Agar

Pseudomonas aeruginosa

Cysteine Lactose Electrolyte Deficient (CLED)

Urinary Pathogens and contaminants

Deoxycholate Citrate Agar (DCA)

Enterics

Eosin Methylene Blue

Grame Negative Coliforms

Hektoen Enteric (HEK) Agar

GIT Pathogens

Lowenstein-Jensen Medium (LJ Medium)

Mycobacteria

Mannitol Salt Agar (MSA)

Staphylococcus aureus

Modified Thayer-Martin Agar

Gonococci

New York City Medium Agar

Gonococci and Genitcal Mycoplasma

Robertson’s Cooked Meat (RCM) medium

Anaerobes

Salmonella-Shigella (SS)

Salmonella and Shigella

Selenite Broth

Salmonella and Shigella

Thioglycollate Broth

Aerobes, anaerobes, microaerobes, facultative anaerobes, fastidious orgnaisms

Thiosulfate Citrate Bile Salts Sucrose (TCBS)

Vibrio Spp

Tinsdale Agar

C. diphtheria

Trans-Isolate (T-I) Medium

CSF Samples

Triple Sugar Iron Agar (TSI) - Bacteria

Enterics

Triple Sugar Iron Agar (TSI) - Sugars

Glucose, Sucrose, Lactose

Tryptic Soy Agar (TSA)

Universal Medium for Both Gram + and Gram -

Xylose Lysine Deoxycholate Agar (XLD)

Enteric Pathogens

Colony Morphology: Shape Descriptors

Circular, filamentous, irregular, Rhizoid (rough), spindle-like, swarming

Colony Morphology: Edge/Margin

Entire, Undulate (wavelike), lobate, erose (jagged), filamentous, curled

Colony Morphology: Surface

Smooth, Dull, Glistening (shiny), waxy, wrinkled

Colony Morphology: Consistency

Dry, moist, wet, butyrous (creamy), mucoid, brittle

Thioglycolate Broth: Gram - Facultative anerobic Bacilli

Even Growth throughout tube

Thioglycolate Broth: Gram + Cocci

Distinct Puffballs

Thioglycolate Broth: Strict Aerobes

Distinct growth at top of tube

Thioglycolate Broth: Strict Anaerobes

Distinct Growth at bottom of tube

Bromothymol Blue: Low End pH and color

6.0 - Yellow

Bromothymol Blue: High End pH and color

7.6 - Blue

Phenol Red: Low End pH and Color

6.4 - Yellow

Neutral Red: High End pH and Color

8.0 - yellow

Neutral Red: Low End pH and Color

6.8 - Red

Phenol Red: High End pH and Color

8.4 - Red

Bromothymol Blue is used in what media?

Cystine Lactose Electrolyte Deficient Agar (CLED), Thiosulfate Citrate Bile Salts Sucrose (TCBS), OF Medium, and Simmons Citrate Agar

Phenol Red is used in what media?

Mannitol Salt, TSI Agar, Urease Test Medium, and Xylose Lysine Deoxycholate Agar (XLD)

Neutral Red is used in what media

MacConkey, Deoxycholate Citrate (DCA), Salmonella-Shigella

Light Source for Fluorescent Microscopes?

Mercury Vapor or Laser

What does the Optical Excitation Filter do?

Isolates one specific wavelengthSe

What does the Dichromic Mirror do?

Separate excitation and emission light in the same light path

Fluorescent Stain: Acridine Orange - How does it work?

Crosses cell membranes and binds nucleic acids (DNA/RNA)

Fluorescent Stain: Auramine-Rodamine - How does it work and what is it used for

Used for Acid Fast staining of Mycobacterium

Works by binding mycolic acid in cell wall

Fluorescent Stain: Calcolour White - What is it used for and how does it work?

Binds Chitin and cellulose

Used in staining fungi

lac Operon: lacZ Function

Beta-Galactosidase that cleaves lactose to form glucose and galactose.

lac Operon: lacY Function

Permease that uptakes lactase

lac Operon: lacA Function

Is a Transacetylase (Unknown Function)

Atrichous

No Flagella

Monotrichous

One flagellum

Amphitrichous

Each (one) flagellum on both ends

Lophotrichous

Bunch at one end

Cephalotrichous

Bunch at both ends

Petrichous

All over the cell

Psychrophile

15 C

Extreme Psycrhophile

5 C

Mesophile

24-40 C

Facultative Thermophile

45-55 C

Obligate Thermophile

70-75 C

Extreme Thermophile

75-80 C

Nutritional Classfication: Order

Energy + Electron Donor + Carbon Source

Energy Source Words?

Photo (Light) and Chemo (Chemical)

Hydrogen/electron Donor Source Words

Organic: Organo
Inorganic: Litho

Carbon Source Words

CO2: Auto

Organic: Hetero

What Antibacterial substances are in Goblet cells?

Lysozyme - Degrade Cell wall
Lactoferrin - Competes for bacterial iron

Lactoperoxidase - Produce Toxic substance to bacteria

Antibody Classes and tidbits about them

IgM: Largest, Pentamer, and 1st to be release
IgG - Second AB released
IgA - Secreted via Fluids

IgE - Allergies and Parasites

IgD - Regulation of AB production

Autoclave: Fastest and Simplest

15 psi, 121 C, 20 minutes

Autoclave: Biologic Waste, broth, solid media

15 PSI, 121 C, 30 minutes
or
132 C for 4 minutes in prevaccuum

Autoclave: Infectious medical Waste

132 C for 30-60 Minutes

Autoclave: Prion Removal

134 C for 18 minutes prevaccuum sterilizer
132 C for 1 hour in gravity displacement autoclave

1 N NaOH for one hour, and then 121 C in gravity autoclave
134 C sterilizer for one hour
1 N NaOH at 121 C for 30 minutes

Pasteurization: Low Temperature Hold (LTH)

63 C for 30 minutes before cooled to 7 C

Pasteurization: High-Temperature Short-Time (HTST)

71.8 C for 15 seconds before cool to 10 C (also called flash pasteurization)

Ultrahigh temperature (UTH)

138 C for at least 25 minutes (extreme pasteurization)

kills all microbes; keep milk in closed sterile container at room temp

BSC Class I

Unsterilized air pass into cabinet, sterilizing only air to be exhausted

BSC Class II: General

Sterilized air to pass into cabinet and to be exhausted from cabinet via vertical laminar flow

BSC Class IIA

Self contained and 70% of air recirculated into work area (mostly used in micro lab)

BSC ClassIIB

Exhaust air discharged outside building (Radioisotopes, toxic chemicals, or carcinogens)

BSC Class III

Completely closed, negative pressure, filtered sterilized air to pass and exhaust from cabinet; for highly contagious pathogens.