Introduction to Chromatography: Quantification of copper in solution

Stationary phase

the solid, gel or immobilized liquid that remains fixed in the column

ex. H+ saturated cation-exchange resin

Mobile phase

The solvent that carries the sample through the stationary phase

ex. DI water

Chromatographic Bed

The support that contains the stationary phase

ex. the glass column packed with the resin

Delivery System

The mechanism that moves the mobile phase through the bed

ex. flow is controlled by gravity,pressure and stopcock

detector

the method used to identify or quanitfy eluted components

ex. titration endpoint

Binding Affinity

Describes how strongly a molecule binds to the stationary phase

Eluent

the mobile phase solvent that moves through the system

Elute

the process of a substance being washed through the stationary phase by the eluent

Eluate

the substance that has been eluted, collected at the end