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A collection of flashcards designed to help review important concepts from the Molecular Spectroscopy lecture.
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What is the primary purpose of a Monochromator in UV-Vis Spectroscopy?
To isolate specific wavelengths of light.
What does the term 'Chromophore' refer to in UV-Visible Spectroscopy?
Molecular groups that absorb radiation in the UV region.
What are the components of a Dual Beam Spectrometer?
It includes a light source, a detector, a sample area, and a reference cell.
In UV-Visible Spectroscopy, what type of bonds do not typically absorb in the UV region?
Sigma (σ) bonds.
What are n electrons and what is their role in UV-Vis Spectroscopy?
They are electrons not involved in bonding; they are excited by UV radiation leading to absorption.
What is the effect of using a Dual Beam Spectrometer compared to a Single Beam Spectrometer?
Dual Beam provides more accurate measurements by minimizing errors from light intensity fluctuations.
Which electronic transitions are most responsible for absorbance in organic compounds?
The presence of pi (π) bonds in conjugated systems.
What adjustments need to be made in a Single Beam Spectrometer during measurements?
Adjusting transmittance to 0% with the shutter closed and to 100% with the blank.
What common application does UV-Visible Spectroscopy serve in the field of Biotech and Pharmaceutical?
It is used for drug binding assays and nucleic acid analysis.
Name one example of a molecule that contains pi (π) bonds and is commonly analyzed using UV-Vis Spectroscopy.
Benzene.