Molecular Spectroscopy Lecture

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A collection of flashcards designed to help review important concepts from the Molecular Spectroscopy lecture.

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10 Terms

1
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What is the primary purpose of a Monochromator in UV-Vis Spectroscopy?

To isolate specific wavelengths of light.

2
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What does the term 'Chromophore' refer to in UV-Visible Spectroscopy?

Molecular groups that absorb radiation in the UV region.

3
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What are the components of a Dual Beam Spectrometer?

It includes a light source, a detector, a sample area, and a reference cell.

4
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In UV-Visible Spectroscopy, what type of bonds do not typically absorb in the UV region?

Sigma (σ) bonds.

5
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What are n electrons and what is their role in UV-Vis Spectroscopy?

They are electrons not involved in bonding; they are excited by UV radiation leading to absorption.

6
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What is the effect of using a Dual Beam Spectrometer compared to a Single Beam Spectrometer?

Dual Beam provides more accurate measurements by minimizing errors from light intensity fluctuations.

7
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Which electronic transitions are most responsible for absorbance in organic compounds?

The presence of pi (π) bonds in conjugated systems.

8
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What adjustments need to be made in a Single Beam Spectrometer during measurements?

Adjusting transmittance to 0% with the shutter closed and to 100% with the blank.

9
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What common application does UV-Visible Spectroscopy serve in the field of Biotech and Pharmaceutical?

It is used for drug binding assays and nucleic acid analysis.

10
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Name one example of a molecule that contains pi (π) bonds and is commonly analyzed using UV-Vis Spectroscopy.

Benzene.