Bio 006 - 15lb: Biotech lab 7

Genomic DNA Extraction

Process of isolating DNA from cells.

Alu Elements

Specific transposable elements in primates' genomes.

Transposons

DNA segments that can move within the genome.

Genetic Locus

Specific location of a gene on a chromosome.

tPA Gene

Gene coding for protein that breaks down blood clots.

PCR

Technique for amplifying DNA in the lab.

DNA Polymerase

Enzyme used in PCR for DNA synthesis.

PCR Master Mix

Contains DNA polymerase, primers, and nucleotides.

Denature

First PCR step; unwinds DNA by heating.

Anneal

Second PCR step; primers bind to DNA.

Extend

Third PCR step; new DNA strands are synthesized.

Thermocycler

Machine used to automate PCR temperature changes.

Allele Frequencies

Proportion of different alleles in a population.

Gel Electrophoresis

Technique for separating DNA fragments by size.

Homozygous Alu -

Genotype with only 100 bp band in PCR.

Homozygous Alu +

Genotype with only 400 bp band in PCR.

Heterozygous

Genotype with both 100 bp and 400 bp bands.

Intron

Non-coding region within a gene.

Exon

Coding region of a gene that expresses protein.

Buffer

Solution used to maintain pH during PCR.

Nucleotides

Building blocks of DNA; A, T, C, G.

Primers

Short DNA sequences that initiate DNA synthesis.

Chelex Tube

Container used for DNA extraction from cells.

Gel Red

Staining dye used to visualize DNA in gels.