heme ref intervals and calculations

WBC ref range

3.6 - 10.6 × 10^9/L

RBC ref range for males

4.20-6.00 x 10^12/L

RBC ref range for females

 3.80-5.20 x 10^12/L

Hb ref range for males

 135-180g/L

Hb ref range for females

120-150g/L

what Hb value classifies as moderate anemia

Hb 70-100g/L

what Hb value classifies as severe anemia

Hb <70g/L

Hct male ref range

 0.400-0.540 L/L

Hct female ref range

0.350-0.490 L/L

MCV ref range

 80.0-100.0 fL (size)

MCH ref range

26-34 pg

MCHC ref range

320-360 g/L (colour)

plt ref range

 150-450 x 10^9/L

RDW ref range

11.5-14.5% (anisocytosis= different sizes)

what is the formula for MCV

 (Hct/RBC) *1000 = fL

What is the formula for MCH

Hb/RBC = pg

what is the formula for MCHC

Hb/Hct = g/L

how would you calculate a leukocyte/ WBC estimate

(under 10x on mic)

[WBC counted / 5] x0.2 = ___ x10^9/L

(ex: (25 wbc/5) x 0.2 = 1x10^9/L

how do you calculate PLT estimate

(under 100x obj on mic)

[PLTS counted/ 5] x 15 = ___x 10^9/L

(ex: (100/5)x 15 = 300 × 10^9/L)

how to do WBC correction with nRBC (calculation: what is formula)

(under 40 x obj on mic)

[100 x WBC COUNT] / [100 + nRBCs out of 100 WBC] = ___ x 10^9/L

(ex: 10 nRBCs, og wbc count= 11.0 ×10^9/L

100 × 11 .0 ×10^9/L / 100+10

= 10 × 10^9/L )

how to do ABSOLUTE reticulocyte count (whats the formula)

(Retic count done on methylene blue stain under 100 x obj)

[RBC COUNT X retics counted per 1000] / 1000] = ___ x 10^9/L

(ex: (3.22 × 100)/ 1000

= 0.32 X 10^12/L

(RBC x 100)

= 3.2 x 10^9/L )

how to do RELATIVE reticulocyte count (whats the formula)

Retics counted per 1000 rbc/ 10 = ___%

(ex: 25 retics from 1000 RBC’s/10

= 2.5%)

whats the formula for blood sample hemocytometer

ONLY CALCULATE WBC AND PLT

cells counted x DF / number of cells counted x 0.1

= ____ 10 × 10^6/L / 1000

= _____ 10 × 10^9/L

whats the formula for body fluid sample hemocytometer

CAN CALCULATE WBC, RBC, PLT

cells counted / number of cells counted x 0.1

= ____ 10 × 10^6/L

(neat dilution.. aka no df)

how to calculate parasitemia

(under 100 x objective)

[# of intracellular organisms / 200 RBCs ] x 100= __%

(ex: count 8 intracellular organisms out of 200 RBCs

(8/200) x 100

= 4%)