8 - Other Stains (Blood & Microorganisms)

1. Benzidine

2. Crystal violet

3. Giemsa

4. Methylene violet

5. Methylene blue

6. Orcein Method

OTHER STAINS (BLOOD):

1. Levaditi’s

2. Warthin Starry

3. Wade-Fite

4. Malachite green

5. Congo red

6. Picric acid

OTHER STAINS (MICROORGANISM):

Benzidine

Is used for detecting hemoglobin, producing a blue to green color.

Crystal violet

Stains platelets and amyloid, resulting in a violet appearance.

Giemsa and Methylene Violet

Are both used for leukocyte differentiation aiding in distinguishing various white blood cell.

Methylene blue

Specifically highlights plasma cells making them more visible under a microscope.

Orcein Method

Is used to detect Hepatitis B surface antigen or HBS antigen, producing a brown to black staining reaction.

Levaditi's Stain and Warthin Starry

This is used for detecting spirochetes, yielding a black appearance.

Wade-Fite technique

Designed for mycobacterium and nocardia, staining them red.

Malachite green

This is used in Kato-katz for detecting helminth eggs and bacterial spores, appearing a green color.

Congo red

Stains the axis cylinders of embryos, though the result color is not specified.

Hemoglobin

Benzidine indication

Blue-green

Benzidine color results

• Platelet 

• Amyloid

  
  

Crystal violet indication

Violet

Crystal violet color results

Leukocyte differentiation

Giemsa indication

Leukocyte differentiation

Methylene violet indication

Plasma cells

Methelyne blue indication

Hepatitis B surface antigen or HBS antigen (HBsAg)

Orcein method indication

Brown-black

Orcein method color results

Spirochetes

Levaditi’s composition

Black

Levaditi’s color indication

Spirochetes

Warthin Starry composition

Black

Warthin Starry color indication

• Mycobacterium

• nocardia

Wade-Fite technique composition

Red

Wade-Fite technique color indication

• Kato-katz (Helmith eggs)

• Bacterial spore

Malachite green composition

Green

Malachite green color indication

Axis cylinders of embryo

Congo red composition