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what does a plant cell include?
cell membrane
nucleus
rough + smooth ER
Ribosomes
golgi body
mitochondria
chloroplasts
cell wall
large permanent vacuole
label plant cell
function of cell membrane
boundary of a cell with its exterior
controls the movement of substances in and out of the cell
receives instructions from other cells
function of nucleus
contains genetic material
function of rough ER
majority of the ER
has ribosomes on surface
folds and packages proteins
proteins are “budded off” in a vesicle and moved to Golgi
function of smooth ER
less abundant
no ribosomes
synthesizes steroid hormones
large amounts found in testicles and ovaries
function of ribosomes
protein synthesis
function of golgi body
chemical modification of proteins, such as adding carbohydrates
produces substances needed for plant cell wall synthesis
produces lysosomes
function of mitochondria
produce ATP by aerobic respiration in animals and plants
cells with high energy requirements have large amounts of mitochondria
function of chloroplast
carry out photosynthesis
label chloroplast
Stroma (in chloroplast)
where light independent stage occurs
Thylakoid membrane (in chloroplast)
where light dependent stage occurs
Grana (in chloroplast)
stacked thylakoid membranes containing photosynthetic pigments; trap light energy
function of cell wall
strength and support for whole plants
preventing cells from bursting due to inflow of water
only in plant cells
made of cellulose
function of large permanent vacuole
in plant cells
stores pigments, water, and waste products
phospholipids
fatty acid tail is uncharged (does not want water to enter)
phosphate head is charged and hydrophilic (wants to enter water and dissolves in water)
carrier proteins
possess specific binding site to which substances bind and change shape as the substance is moved across the membrane
channel proteins
a pore through which substances will pass through
fluid mosaic model labeled
active transport
uses energy to pass molecules through carrier proteins
passive transport
diffusion
no additional energy input (small and non-polar like O2 and CO2 diffuse through phospholipid bilayer between fatty acid tails)
labeled animal cell
how to calculate magnification of an image
measure the length of the image with a ruler
ensure to have the same units for the image length and actual length
use the formula for magnification by dividing the image length by the actual length
diffusion
the movement of molecules from high to low concentration (passive process = no additional input of energy)
facilitated diffusion
movement of molecules from high to low concentration through carrier or channel proteins
osmosis
diffusion of water (from higher to lower water potential)
active transport
using energy (ATP) to move molecules across the cell membrane
carrier proteins bind molecules to one side of membrane
atp binds to carrier protein and is broken down to ADP and phosphate
releases energy
energy is used to change shape of protein to move the molecule across the membrane
returns to original shape
water potential
potential energy of water in a solution compared to pure water; water will move by osmosis from high to low water potential
the more water molecules, the higher the water potential
if a solute such as salt is dissolved in water, the proportion of water molecules in the solution decreases water potential
hypertonic
solution has greater concentration of solutes
lower water potential than the cells/body fluids of an organism
isotonic
solution has greater concentration of solutes
lower water potential than the cells/ body fluids of organism
hypotonic
solution has lower solute concentration
higher water potential than cells/body fluids of organism
smaller organisms/cells have a larger or smaller SA:volume ratio?
larger
Larger SA:Volume ratio =
faster rate of diffusion
why do larger organisms need gills or lungs
for diffusion
increased surface area (like tentacles or coral polyps) =
increased area through which gases can diffuse
gills
gill arches hold gill filaments
gill filaments have a number of lamellae
lamellae increase the gas exchange surface area
Gill hyperplasia
Shortening, rounding, and fusion of gill lamellae
increases mucus production
reduces surface area for gas exchange
circulatory system in a typical fish
blood passes through muscles and other body tissues and releases O2→picks up Co2
blood pumped back to the heart (through veins)
blood pumped from heart to gills (through arteries)
blood passes through gills (capillaries) + releases CO2 and picks up O2
Blood leaves gills (in arteries) and travels to muscles and other body tissues
to deliver O2 and remove CO2
what organisms use simple diffusion
small animals and/or animals with thin/few tissue layers
coral polyps
pumped ventilation
demersal and slow swimming fishes (ex. grouper)
Open mouth, close operculum, creates a low pressure in the buccal cavity, draws water in
close mouth, open opercula, increases pressure in the buccal cavity, pushes water out through the gills (diffuse O2 in and CO2 out)
ram ventilation
pelagic fishes (constant swimmers; have higher o2 demand) ex. tuna
swim continuously, with mouth open, so water constantly flows in through the mouth and over the gills to diffuse O2 in and CO2 out
why do marine organisms need to regulate their water content and ion content
if they live in an environment with high salinity and low water potential
osmoconformer
organism with the same internal solute concentration/water potential as surrounding water
ex. mussels
when salinity changes, mussels close shells to prevent seawater coming into contact with body tissue
can increase and decrease solute concentrations of their cells if external salinity changes
osmoregulator
organism that maintains internal solute concentration (body fluids)
Ex. salmon, tuna, bull shark
salmon migrate from freshwater to the ocean and maintain their internal solute concentration
tuna live off shore their entire lives, where salinity doesn’t change
bull sharks (juveniles) migrate from the ocean into freshwater rivers
euryhaline
organisms that can tolerate a WIDE range of salinities
ex. salmon, bull sharks, and mussels
stenohaline
organisms that can tolerate only a NARROW range of salinities
ex. tuna
Osmoregulation characteristics in marine fishes
surrounding water typically has higher salinity than their cells and body fluids
constantly drink seawater to replace water lost by osmosis
Sodium and chlorine ions are actively secreted by the gills. Specialized cells on the gill filaments have protein “pumps” that pump ions into the water; uses ATP
magnesium and sulfate ions are actively secreted by the kidney into urine
reabsorption of water by the kidney produces a low volume of very concentrated urine
osmoregulation characteristics in freshwater fishes
surrounding water has lower salinity and higher water potential than their cells and body fluids
drink small amounts of water
gills actively pump sodium and chloride ions into blood and body fluid. specialized cells have protein pumps that actively pump the ions from the external water to the internal body fluids; uses ATP
produce large amounts of very dilute urine
electromagnetic spectrum
from left to right:
gamma rays
x-rays
ultraviolet light
visible light
infrared
radio waves
light wavelength
the wavelength/visible color of light in nm
light intensity
measure of strength or brightness of light
light penetration
depth at which light can penetrate through water
red light
penetrates into the most shallow waters - down to 10 m
as it is absorbed by the surface water
longest wavelength (620-720nm)
blue light
penetrates the deepest - down to 200 m
short wavelength
green light
penetrates depths deeper than red, but shallower than blue
photosynthesis number equation
6co2 + 6H20 → C6H12O6 + 6O2
light dependent stage
takes place in grana of the thylakoid membranes
trap light energy and transform into chemical energy used to produce glucose
grana contain primary and accessory pigments embedded in their membranes in clusters called photosystems
when chlorophyll a (primary pigment) absorbs light energy it undergoes photoactivation
the molecule loses an electron; it is oxidised (losing electrons: oxidation, gaining electrons: reduction)
b/c the electron loss was due to the trapping of light (photooxidation)
summary of light-dependent stage
function : harvest light energy and convert to chemical energy
energy is given to 2 molecules, ATP and reduced NADP
photolysis of water provides electrons to replace those lost by oxidized chlorophyll a
oxygen is released as a byproduct of photolysis
light-independent stage
2nd stage of photosynthesis
the energy (ATP and reduced NADP) that has been harvested in the light dependent stage is used to make glucose by carbon dioxide fixation
takes place in the stroma of the chloroplast
calvin cycle makes glucose
carbon containing molecules are converted through a range of different forms
calvin cycle
carbon dioxide is combined with RuBP by enzyme rubisco
Joining carbon dioxide and RuBP makes a sugar with 6 carbon atoms which breaks into GP
GP is converted into a diff sugar called TP which produces glucose from ATP and NADPH
some of the TP is used to make glucose while the rest is used to make new sugar called RuP
RuP is given another phosphate by more ATP to make RuBP. The RuBP can then repeat the calvin cycle with fresh carbon dioxide
light independent stage summarized
uses the energy from ATP and reduced NADP that were produced in the light dependent stage
enzyme rubisco combines carbon dioxide with RuBP
it fixes carbon dioxide and makes glucose
energy from light ends up in the glucose
chloroplast pigments
absorbs blue and red
organisms that are green live in the shallowest depths (to absorb red light)
carotenoids absorb blue
organisms that are yellow/brown/orange live deeper
fucoxanthin absorbs blue and green
phycocyanin (blue-green) absorbs orange, yellow and green
Phycoerythrin absorbs blue and green
green algae and seagrass live in
shallowest water
blue-green (contains phycocyanin) bacteria live in
deeper water
brown/yellow/orange algae (kelp, dinoflagellates, etc) live
deeper than green
red algae lives
in the deepest, but still shallow water bc they need sun for photosynthesis
red and brown algae live
at deeper depths
why do pigments live at varying depths
based on their absorption spectra to REDUCE COMPETITION
effect of light intensity on rate of photosynthesis
increases then stabilizes bc another factor becomes limited
effect of carbon dioxide concentration on rate of photosynthesis
increases then stabilizes bc another factor becomes limiting
effect of temperature on the rate of photosynthesis
increases then falls bc enzymes denature
chemosynthesis
the fixation of carbon using the chemical energy of dissolved substances
chemosynthesis formula
12H2S + 6CO2 → C6H12O6 + 6H2O + 12S
hydrogen sulfide + carbon dioxide → glucose + water + sulfur
endoriftia and riftia
mutualism
endoriftia lives in riftia’s trophosome
using their plume (external gills) riftia takes in H2S for Endoriftia to chemosynthesize
Endoriftia produce glucose and other organic molecules
Endoriftia get constant supply of H2S for chemosynthesis
aerobic respiration
energy is released from glucose by oxidation, producing carbon dioxide and water as waste products
aerobic=uses oxygen
energy released is ATP
begins in cytoplasm, continues in mitochondria
1 molecule of glucose produces up to 38 molecules of ATP
anaerobic respiration
without oxygen
incomplete combustion of glucose
1 glucose molecule generates only 2 molecules of ATP
occurs in cytoplasm