Effects of RET/GFRA receptors in EEC Cell Research Project

what does RET signaling do to the release of GLP-1 and PYY?

inhibits

what are the 4 RET ligands?

GDNF, NRTN, ARTN, persephin

how do RET ligands signal RET downstream signaling?

RET ligands bind to GFRA receptors to activate RET receptors to initiate downstream signaling

which RET co-receptor is highly expressed by mouse EECs? and is highly expressed by which population?

GFRA3, “immature” EEC poulations

RET signaling is mediated by which signaling

GDNF-Gfra1 not ARTN-Gfra3

main purpose of the study

compare effect of different RET ligands on murine and human EECs

Is it possible for GDNF-GFRA family to singal NCAM1? (random tangent to keep in mind)

can signal NCAM1 along with RET, but stronger affinity to RET. Not believed to be expressed in EECs

two possibilities of RET signaling of RET and GFRA receptors

in trans (different cell), in cis (same cell)

in trans RET signaling

GFRA receptors are expressed on different cell as RET to initiate downstream signaling (ex: immune cell vs. EEC)

in cis RET signaling

GFRA recpetors are expressed on the same cell as RET to initiate downstream signaling

study 1.1

access level of RET, GFRA1-4 and NCAM1 in STC-1 cells

STC-1 cells

immortalized cell line of EECs in murine tumor

study 1.1 procedure

protein stain GFRA1 and GFRA3, mRNA of RET, GFRA1-4 and NCAM1 with qPCR

study 1.2

access level of RET, GFRA1-4 and NCAM1 in human intestinal epithelial