hema lab - midterms

Evaluated Tube System

• Two-way Needle

• Adapter

Tourniquet

Seraket

Sealant (seal ease)

Name all parts

Butterfly Infusion Set

Name:

A. Left

B. Right

A. Heparinized Capillary Tube

B. Plain Capillary Tube

Automatic Lancet

Surgicutt

Needles for syringe: Color & Gauge size

• 1

• 2

• 3

Hub Cutter

What & Use

Feather Lancet

used to deliver a precise amount of puncture and depth primarily utilized when measuring bleeding time and clotting time.

What & Use

Ordinary Lancets

intended to puncture the skin to produce blood for a specific test

• Longer ones: Adults

• Smaller ones: Pediatric patients

Where to skin puncture

• 3rd/ 4th fingers on the palmar side of the skin of the non-dominant hand

• the puncture site should be perpendicular to the ridges of the fingerprint

Name & most preferred to least

TRUE OR FALSE:

Patient should be instructed to pump the fist for better palpation of the vein.

FALSE

Patient should NOT be instructed to pump the fist: this could lead to

hemoconcentration.

ORDER OF DRAW:

1. ETS METHOD

2. SYRINGE METHOD

3. DERMAL PUNCTURE

Yellow Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Light Blue Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Black Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Red (Glass) Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Red (Plastic) Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Gold / Tiger Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Green / Light Green Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Lavender Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

White / Pearl Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Pink Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Tan / Brown Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Royal Blue Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Gray Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

Orange Tube

• Additive

• Inversions

• Lab Section

• Lab Tests

ERYTHROPOIESIS

Pronormoblast / Proerthrocyte / Rubriblast

Stage lasts: >24 hours

ERYTHROPOIESIS

Basophilic normoblast / Basophilic erythroblast / Prorubricyte

Stage Lasts: >24 hours

ERYTHROPOIESIS

Polychromatophilic normoblast / polychromatophilic erythroblast / Rubricyte

Stage Lasts: ~30 hours

ERYTHROPOIESIS

Orthochromatic normoblast / orthochromatic erythroblast / Metarubricyte

Stage lasts: 48 hours

ERYTHROPOIESIS

Polychromatic (Polychromatophilic) Erythrocyte / Diffusely basophilic erythrocyte / RETICULOCYTE

24-48 hours

ERYTHROPOIESIS

Erythrocyte / Normal RBC / Discocyte

ABNORMAL CELLS

Macrocyte

ABNORMAL CELLS

Microcyte

ABNORMAL CELLS

Target Cells

ABNORMAL CELLS

Sickle Cells

ABNORMAL CELLS

Spherocyte

ABNORMAL CELLS

Stomatocyte

ABNORMAL CELLS

Schizocyte

ABNORMAL CELLS

Rouleaux formation

ABNORMAL CELLS

Howell-Jolly bodies

Name stage of erythropoiesis

What stain ?

Reticulocyte

STAIN: Brilliant Cresyl Blue (Supra vital stain)

Name stage of erythropoiesis

What stain ?

Reticulocyte

Stain: Wright Srain

?

Platelets on Top of RBC or Thrombocyte

What is Hgb?

Sahli hemoglobinometer with graduated tube

anticoagulated blood to mark 20 cu mm, (20ul/0.02mL)

Reaction time?

Reported in?

Reaction time: 10 minutes for color development

Reported in: Grams %

what method?

Wavelength?

Reaction Time?

Reported in?

Cyanmethemoglobin Method

Wavelength: 540nm

Reaction time: 10 minutes to allow full conversion to cyanmethemoglobin

Reported in: grams of hemoglobin per deciliter (g/dL)

Cyanmethemoglobin Method

Principle Reaction?

TRUE / FALSE

In Cyanmethemoglobin Method, The absorbance of the solution is directly proportional to the amount of hemoglobin present.

TRUE

Drabkin’s solution composition?

Microhematocrit centrifuge

Microhematocrit reader device / Spirocrit

RBC Pipette

Composed of?

Hayem’s solution:

• Mercuric chloride (0.5 g)

• Na2SO4 (5 g Sodium sulfate)

• NaCl (1 g)

• Distilled water (200 mL)

Hemacytometer with improved Neubauer ruling and coverglass

Cell counter

Rubber sucking tube

Pipette shaker