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Aseptic Technique
Methods used to stop the growth and reproduction of microorganisms to prevent infection.
Father of Aseptic Technique
Joseph Lister.
Goal of Infection Prevention
Achieve asepsis (absence of pathogenic microorganisms).
Health Care-Associated Infection (HAI)
An infection that develops during a patient's stay in a health care facility.
Community-Acquired Infection
An infection acquired outside of a health care facility.
Medical Asepsis
Clean technique that inhibits the growth and transmission of pathogens.
Examples of Medical Asepsis
Hand hygiene, changing linens, routine patient care.
Surgical Asepsis
Sterile technique that destroys all microorganisms and spores.
Examples of Surgical Asepsis
Operating room procedures, catheter insertion, sterile wound care, injections.
Chain of Infection
The six steps required for an infection to spread.
6 Links of the Chain of Infection
Infectious agent, reservoir, portal of exit, mode of transmission, portal of entry, susceptible host.
Infectious Agent
A microorganism capable of causing disease.
Pathogen
A disease-causing microorganism.
Reservoir
The place where microorganisms live and multiply.
Portal of Exit
The path by which pathogens leave the reservoir.
Mode of Transmission
How pathogens spread from one place to another.
Portal of Entry
The route pathogens use to enter a susceptible host.
Susceptible Host
A person who cannot effectively resist infection.
Bacteria
Single-celled microorganisms that may cause disease.
Aerobic Bacteria
Bacteria that require oxygen to grow.
Anaerobic Bacteria
Bacteria that grow without oxygen.
Bacterial Spores
Highly resistant forms of bacteria that survive harsh conditions.
Capsule (Bacteria)
Protective layer that helps bacteria resist antibiotics.
Gram Stain
Test used to identify bacteria as gram-positive or gram-negative.
Viruses
The smallest infectious agents that require host cells to reproduce.
Do Antibiotics Treat Viruses?
No.
Viral Treatment
Usually symptom management; some require antiviral medications.
Fungi
Yeasts and molds that can cause infections.
Common Fungal Infection Sites
Skin, hair, nails, and mucous membranes.
Protozoa
Single-celled parasites that may infect the GI, GU, respiratory, or circulatory systems.
Example of Protozoal Disease
Malaria.
Common Reservoirs
Humans, animals, water, soil, contaminated surfaces.
Common Portals of Exit
Respiratory secretions, blood, body fluids, open wounds.
Types of Transmission
Contact, droplet, airborne, vector, fomites.
Fomite
An object that carries infectious microorganisms.
Common Portals of Entry
Mucous membranes, respiratory tract, urinary tract, broken skin.
Factors Increasing Susceptibility
Age, chronic illness, immunosuppressants, stress, fatigue, poor nutrition.
Common HAIs
Surgical site infections, UTIs, pneumonia, bloodstream infections.
Role of Infection Control Nurse
Monitors infections and develops infection prevention practices.
Occupational Health Service
Protects healthcare workers and patients through infection prevention.
Hepatitis B Vaccine Requirement
Employers must provide it to workers at risk for occupational exposure.
Needlestick Injury
Response
Most Common Infection from Needlesticks
Hepatitis B.
Standard Precautions
Practices used with all patients to prevent disease transmission.
When to Perform Hand Hygiene
Before and after patient contact, after contaminated items, and when hands are visibly soiled.
How Long Should You Wash Hands?
15–20 seconds.
When Can Alcohol Hand Sanitizer Be Used?
When hands are not visibly dirty.
Standard Precautions Apply To
Blood, body fluids except sweat, nonintact skin, and mucous membranes.
Personal Protective Equipment (PPE)
Gloves, gowns, masks, and eye protection used to reduce exposure.
Sharps Safety
Never recap needles; dispose of them in approved sharps containers.
Preventing Cross-Contamination
Provide personal care items, teach hand hygiene, separate contaminated equipment.
Transmission-Based Precautions
Additional precautions for patients with known or suspected infections.
Three Types of Transmission-Based Precautions
Airborne, Droplet, Contact.
Airborne Precautions
Used for infections spread by tiny particles that remain suspended in the air.
Diseases Requiring Airborne Precautions
Measles, tuberculosis, COVID-19, chickenpox.
Airborne Isolation Room
Negative-pressure room with door kept closed.
Respirator Required for Airborne Precautions
N95 respirator or PAPR.
Fit Test
Determines correct respirator size and seal.
Fit Check
Performed every time a respirator is worn.
Droplet Precautions
Used for infections spread by large respiratory droplets.
Examples Requiring Droplet Precautions
Meningitis, pneumonia caused by Haemophilus influenzae or Neisseria meningitidis.
Contact Precautions
Used for infections spread through direct or indirect contact.
Diseases Requiring Contact Precautions
C. difficile, RSV, Hepatitis A, E. coli O157:H7, herpes simplex.
Cleaning
Removal of dirt, debris, and organic matter.
Disinfection
Process that destroys microorganisms but not spores.
Sterilization
Process that destroys all microorganisms, including spores.
Antiseptics
Agents that inhibit microorganism growth and are safe for human tissue.
Disinfectants
Chemicals used on inanimate objects to kill microorganisms.
Examples of Antiseptics
Alcohol and chlorhexidine (CHG).
Examples of Sterile Procedures
Urinary catheterization and sterile dressing changes.
Sterile Body Areas
Blood, cerebrospinal fluid, internal organs, and bone.
Sterile Technique Rule
Sterile only touches sterile.
If Sterile Touches Nonsterile
It becomes contaminated.
Requirements for Sterile Packages
Intact, unopened, dry, and undamaged.
Where Should Sterile Items Be Kept?
In sight and above waist level.
What Contaminates a Sterile Field?
Turning away, reaching over it, moisture, touching nonsterile objects.
Moisture and Sterility
Moisture contaminates sterile items because microorganisms travel through wet surfaces.
Gravity Rule in Sterile Technique
Fluids flow downward; dirty fluid contaminates sterile surfaces.
Sterile Field Border
The outer 1-inch border is considered contaminated.
Purpose of Infection Prevention
Protect patients, healthcare workers, and reduce spread of infection.
Why Are Hospitalized Patients at Higher Risk?
Illness, weakened immunity, invasive procedures, and exposure to pathogens.
Most Important Method to Break the Chain of Infection
Hand hygiene.