microscopy calculations

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Last updated 1:03 PM on 9/19/26
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4 Terms

1
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calculating magnification using scale bars

measure the length of the scale bar in mm

convert to same units as scale bar label

divide length (image scale bar length) by the label (actual scale bar length)

2
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calculating specimen size using scale bars

measure length of specimen in mm

measure length of scale bar in mm

divide specimen by scale

multiply this constant with the scale bar label

3
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calculating specimen size using magnfication

measure length of specimen in mm

convert to micrometers

divide specimen length by magnification

4
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resolution

the ability to distinguish two separate objects in an image