1/27
These flashcards cover vocabulary and definitions from the lecture notes regarding the identification of gram-negative rods and their antibiotic susceptibility.
Name | Mastery | Learn | Test | Matching | Spaced |
---|
No study sessions yet.
Enterobacteriaceae
A family of bacteria commonly found in the guts that includes Escherichia coli, Pseudomonas aeruginosa, Enterobacter sp., Proteus, and Salmonella.
TSA (Tryptic Soy Agar)
A non-selective medium that supports the growth of many different microbes.
Selective Media
Media that inhibits the growth of all bacteria except for the desired bacteria.
Differential Media
Media that allows visualization of physical differences between the organisms that are growing.
Fastidious Organisms
Organisms that require many growth factors to be added to culture media.
Enrichment Media
Media that contains extra compounds to support the growth of fastidious organisms.
Oxidase Test
A test for the presence of the enzyme cytochrome oxidase; indicated by a color change.
MacConkey Agar
A selective and differential medium for gram-negative bacteria that differentiates between lactose fermenters and non-fermenters.
Citrate Agar
Tests for an organism's ability to utilize citrate as the sole carbon source, indicated by a color change to blue.
Phenylalanine Deaminase Test (PDA)
Tests for the enzyme phenylalanine deaminase; indicated by a green color after adding ferric chloride.
Colony
A visible area of microbe growth arising from a single original cell.
Generation Time
The time it takes for a population to double, starting from one cell.
Bactericidal
Antibiotics that kill bacteria.
Bacteriostatic
Antibiotics that slow down the growth and multiplication of bacteria.
Zone of Inhibition
The area around an antibiotic disk where bacteria cannot grow, indicating susceptibility.
Kirby-Bauer Test
A disc diffusion method used to determine antibiotic susceptibility.
Resistance Mechanism
Adaptations bacteria have to survive in the presence of antibiotics, e.g., through plasmid acquisition.
Aseptic Technique
Laboratory methods to prevent contamination from pathogens.
MacConkey Function
Selective for gram negatives and differentiates lactose fermenters (pink) from non-fermenters (clear).
KIA (Kligler’s Iron Agar)
Tests the ability to ferment lactose and/or glucose and produce hydrogen sulfide.
Antibiotic Resistance
The ability of bacteria to resist the effects of antibiotics, often due to genetic adaptations.
Plasticity of Bacterial Genome
The capability of bacteria to rapidly adapt to changing environments, including antibiotic exposure.
Misuse of Antibiotics
Failure to follow proper instructions for antibiotic use, leading to resistance.
PDA Color Change
A positive result in a phenylalanine deaminase test is indicated by a green color.
Antibiotic Susceptibility Testing
A method to determine which antibiotics are effective against particular bacterial infections.
Muller-Hinton Agar
A type of agar used for antibiotic susceptibility testing where discs are placed to observe bacterial growth inhibition.
Inhibition of Cell Wall Synthesis
One of the targets for antibiotics, including penicillins and cephalosporins.
Importance of Labeling
Crucial in laboratory settings to track organisms and avoid cross-contamination.