ENTERO + BIOCHEMICAL (2)

Gram negative

Sodium azide may be added to culture media to inhibit:

Oxidase

Enzymes not named on the basis of function:

Pseudomonas

Oxidase positive organism:

Positive coagulase test

An identifying characteristic of Staphylococcus aureus:

Virulence

Capsules are associated with:

Amines

Decarboxylation of the amino acids lysine, ornithine, and arginine result in the formation of:

Bacillus subtilis

“Hay bacillus”:

Clostridium tetani

“Tack-head” bacillus:

Factor V

A heat-labile factor present in blood known as nicotinamide adenine dinucleotide which is an important energy carrier in oxidation-reduction systems:

Factor X

A heat-stable factor present in blood known as hemin that is important to the production of respiratory enzymes such as cytochrome oxidase, catalase, and peroxidase:

Listeria monocytogenes

A small gram-positive rod that causes meningitis and septicemia in neonates:

Indole

A tryptophan broth culture produces a red color following the addition of Ehrlich's reagent. The color produced indicates the presence of:

Lipid-rich cell wall

Acid-fast staining of Mycobacterium is attributable to:

Ziehl-Neelsen method

Acid-fast staining used to differentiate M. lacticola and M. smegmatis from M. tuberculosis (red acid-fast bacilli):

Positive

Actinomyces, Streptomyces, Saccharomyces (yeast and mold) are Gram:

Listeria

All are Gram-negative bacilli, except:

Peptostreptococcus, Peptococcus, Coprococcus, Ruminococcus

All are Gram-negative cocci except:

N. asteroides

All are Gram-negative cocci, except:

Dorset egg medium

All are selective media for cultivation of M. tuberculosis, except:

A. Oleic acid albumin agar
B. Middlebrook 7H-10 agar & Petragnani
C. Lowenstein–Jensen
D. Dorset egg medium

Campylobacter

All bacilli are gram-positive except:

20% potassium hydroxide

All can be used as digesting substances for concentration of TB bacilli, except:
A. N-acetyl-L-cysteine + 40% NaOH
B. 4% NaOH
C. 4% H₂SO₄
D. 20% potassium hydroxide

5g

Amount of phenol crystals added in Kinyoun Acid-Fast Staining:

Corynebacterium

Bacteria that require enriched media, except:

Pleomorphic

Bacterial cells of one species which vary in their size and arrangement are said to be:

Corynebacterium diphtheriae

Capsulated organism, except:
A. B. anthracis
B. K. pneumoniae
C. S. pneumoniae
D. Corynebacterium diphtheriae

Preventing phagocytosis

Capsule increases virulence by:

3%

Concentration of H₂O₂ used in Catalase test:

1 & 3

The following biochemical results are TRUE for Klebsiella pneumoniae, except:

1. Citrate: BLUE 2. LIA: K/A 3. Motility: negative 4. VP: negative

1 & 3

On BAP, S. aureus demonstrates the following colonial characteristics, except:

1. Pinpoint 2. Smooth 3. White 4. Butyrous

1 & 3

The following biochemical results are TRUE for Staphylococcus aureus, except:

1. OF tube Test: Open tube–Green, Closed tube–Yellow 2. Bacitracin: Resistant 3. Mannitol fermentation: Red 4. Novobiocin: Susceptible

1, 2, 3

Which of the following is/are true biochemical test result/s for Citrobacter freundii?

1. TSI: K/A after 48 hours 2. H₂S Production: with Blackening 3. Indole: Red 4. ONPG: Yellow

1 ONLY

The following organism/s is/are able to produce pigmentation on plated media, except:

1. Staphylococcus epidermidis 2. P. aeruginosa 3. S. marcescens 4. S. pyogenes

1, 2, 3

The following organism/s is/are citrate utilization test positive, except:

1. Shigella sonnei 2. Proteus vulgaris 3. Salmonella typhi 4. Providencia rettgeri

2 & 4

The following are true biochemical results of Proteus mirabilis, except:

1. TSI: K/A + H₂S 2. Urease test: Amber 3. LIA: K/A + H₂S 4. Motility: Motile

1 & 3

The following work-up results will help the MT in identifying Escherichia coli, except:

1. Growth on Mac: pink dry colonies 2. PAD: yellow 3. MR: red 4. LDC: purple

2 & 4

The following genus/es will give a yellow-orange result in urease test within & after 4 hours of incubation, except:

1. Enterobacter 2. Proteus 3. Klebsiella 4. Morganella

1, 2, 3

The following organism/s has/have a K/K reaction on LIA, except:

1. Enterobacter cloacae 2. Serratia marcescens 3. Citrobacter koseri 4. Klebsiella pneumoniae

3 ONLY

Which of the following reagents are used for the detection of acetoin?

1. KOH 2. Alpha naphthylamine 3. Alpha naphthol 4. Sulfanilic acid

1, 3, 4

What sugars are incorporated in the TSI media?

1. Sucrose 2. Maltose 3. Glucose 4. Lactose

1 ONLY 

Escherichia coli serves as the POSITIVE CONTROL for which of the following tests?

1. Indole production 2. Methyl red test 3. Acetamide utilization 4. MUG test

1, 2, 3, 4

Detection of H₂S can be made on which media?

1. TSI 2. LIA 3. SIM 4. HEA

1, 2, 3, 4

Which of the following are considered as diagnostic tests for Gram-positive bacteria?

1. Catalase test 2. Coagulase test 3. Modified oxidase test 4. Bacitracin disk test

2 & 4

All of the following are FALSE regarding Coagulase test EXCEPT:

1. S. aureus is the positive control for this test 

2. The bacterial suspension should be mixed with distilled water

3. The slide method detects the clumping factor which reacts with fibrinogen

4. S. epidermidis is the positive control for this test

1 ONLY

Which of the following is/are TRUE regarding PYR test?

1. This test detects the presence of the enzyme L-pyrroglutamylaminopeptidase

2. Positive result is the development of a bright red color after 5 minutes

3. The β-naphthylamine produced is detected in the presence of N, N-methylaminocinnamaldehyde

4. Enterococcus faecium is the positive control for this test

1, 2, 3, 4

Which of the following is incorporated in LIA?

1. Peptones 2. Ferric ammonium citrate 3. Sodium thiosulfate 4. Glucose

1, 2, 3

Pseudomonas aeruginosa is the POSITIVE CONTROL for which of the following tests?

1. Bacitracin test 2. Citrate utilization 3. MUG test 4. Acetamide utilization

1 ONLY

TRUE regarding Citrate Utilization:

1. Used to determine ability of the organism to utilize inorganic ammonium salts as its only nitrogen source

2. The indicator incorporated in this medium is methylene blue

3. Escherichia coli is positive in this test

4. Blue is the color of the uninoculated media

1 & 3

The following organism/s demonstrate/s “fried egg colonies” on media:

1. Eikenella corrodens 2. Mycoplasma pneumoniae 3. Chromobacterium violaceum 4. Fusobacterium mortiferum

1, 2, 3, 4

End products of carbohydrate fermentation in TSI:

1. Acid 2. Gas 3. Alcohol 4. H₂S